passages.id: split_1_test_2994_passage
passages.type: progene_text
passages.text: alpha-1,3-Glucanase , which was isolated from an ammonium sulfate fraction of 0 - 30 % saturation of KA - prep , gave the protoplast - forming activity to an ammonium sulfate fraction of 30 - 50 % saturation of KA - prep , which contained chitinase ( s ) and beta - glucanase ( s ) but was inactive in the protoplast formation .