passages.id: split_1_test_2995_passage
passages.type: progene_text
passages.text: Chitinase(s) and beta - glucanase ( s ) in the ammonium sulfate fraction of 30 - 50 % saturation were separated by DEAE - cellulofine A - 500 column chromatography , and the protoplast - forming activity appeared when the chitinase preparation was mixed with the alpha-1 , 3 - glucanase .