passages.id: split_1_test_1065_passage
passages.type: progene_text
passages.text: The beta subunit cDNA was cloned into the pQE-30 expression vector , and the recombinant His tagged protein , which was expressed in Escherichia coli , was recognized by antibodies to the human MAT II , to synthetic peptides copying the sequence of native beta subunit protein , and to the rbeta protein .