passages.id: split_1_test_1934_passage
passages.type: progene_text
passages.text: Culture of an H-2(s) - restricted , bovine myelin basic protein ( BMBP ) - specific murine Th1 clone with the adenyl cyclase agonist forskolin ( FSK ) or isobutylmethylxanthine ( IBMX ) , an inhibitor of cAMP catabolism , before culture with anti - CD3 or BMBP and antigen - presenting cells ( APC ) suppressed antigen or anti - CD3 - induced proliferation and production of interferon-gamma ( IFN-gamma ) .