passages.id: split_1_test_707_passage
passages.type: progene_text
passages.text: However , at their native locations , where the two ARSs are in a cluster with ARS302 , the I silencer , no replication origin activity was detected regardless of yeast mating type , special growth conditions that induce the transcriptionally repressed CHA1 gene , trans - acting mutations that abrogate transcriptional silencing at HML ( sir3 , orc5 ) , or cis - acting mutations that delete the E and I silencers containing ARS elements .