passages.id: split_1_test_2816_passage
passages.type: progene_text
passages.text: Pseudo - initiation complex dissociation was impaired by substitution of Arg99 and Arg112 ( whose positive charges are important ) and , to a lesser extent , of Arg116 , Arg129 , Arg133 and Arg147 , while the dissociation of non - canonical 30S initiation complexes was preserved at wild - type levels in all 32 mutants .