Datasets:

taln-ls2n

/

kpbiomed

like 5

Follow

TALN research group at LS2N lab 18

30900806

Estimating the Mass of Chemicals Associated with Ocean Plastic Pollution to Inform Mitigation Efforts.

Plastic pollution in the marine environment is well documented. What remains less recognized and understood are the chemicals associated with it. Plastics enter the ocean with unreacted monomers, oligomers, and additives, which can leach over time. Moreover, plastics sorb organic and inorganic chemicals from surrounding seawater, for example, polychlorinated biphenyls (PCBs) and metals. Thus, interception and cleanup of plastics reduces the amount of chemical contaminants entering or reentering the oceans and removes those already present. Here, we estimate 1) the mass of selected chemical additives entering the global oceans with common plastic debris items, and 2) the mass of sorbed chemicals (using PCBs as a case study) associated with microplastics in selected locations. We estimate the mass of additives that entered the oceans in 2015 as constituents of 7 common plastic debris items (bottles, bottle caps, expanded polystyrene (EPS) containers, cutlery, grocery bags, food wrappers, and straws or stirrers). We calculate that approximately 190 tonnes (t) of 20 chemical additives entered the oceans with these items in 2015. We also estimate the mass of PCBs associated with microplastics in 2 coastal (Hong Kong and Hawaii) and 2 open ocean (North Pacific and South Atlantic gyres) locations, as comparative case studies. We find that the mass of chemicals is related to the mass of plastics in a location, with greater mass of PCBs closer to the source (i.e., land), where there is more plastic per unit area compared to the open ocean. We estimate approximately 85 000 times more PCBs associated with plastics in an average 4.5-km stretch of beach in Hong Kong than from the same size transect in the North Pacific gyre. In conclusion, continuing efforts for plastic interception and cleanup on shorelines effectively reduces the amount of plastic-related chemicals entering and/or reentering the marine environment. Integr Environ Assess Manag 2019;15:596-606. © 2019 SETAC.

['De Frond|Hannah L|HL|', 'van Sebille|Erik|E|', 'Parnis|J Mark|JM|', 'Diamond|Miriam L|ML|', 'Mallos|Nicholas|N|', 'Kingsbury|Tony|T|', 'Rochman|Chelsea M|CM|']

2019

25843626

Oncolytic measles virus as a novel therapy for malignant peripheral nerve sheath tumors.

Malignant peripheral nerve sheath tumors (MPNSTs) are devastating soft tissue sarcomas that can arise sporadically or in association with neurofibromatosis type I, have a poor prognosis, and have limited treatment options. Oncolytic measles virus therapy has been demonstrated to have significant antitumor properties in a number of different cancers, but the oncolytic potential of a MV Edmonston (MVEdm) vaccine strain engineered to express the human sodium iodide symporter (MV-NIS) on MPNST has not previously been evaluated. MPNST cell lines were found to highly express CD46, a cellular receptor required for measles viral entry, on their cell surface. After in vitro MV-NIS infection, MPNST cell lines showed significant cytopathic effect (CPE), while normal Schwann cells were less susceptible to CPE. Virus localization and distribution could be monitored by imaging of I-125 uptake. Local administration of MV-NIS into MPNST-derived tumors resulted in significant regression of tumor and improved survival. These results demonstrate feasibility of oncolytic measles virus therapy for MPNST patients and the possibility of a novel treatment for patients with NF1 tumors.

['Deyle|David R|DR|', 'Escobar|Diana Zarate|DZ|', 'Peng|Kah-Whye|KW|', 'Babovic-Vuksanovic|Dusica|D|']

2015

33637131

Community-driven citizen science approach to explore cardiovascular disease risk perception, and develop prevention advocacy strategies in sub-Saharan Africa: a programme protocol.

BACKGROUND In sub-Saharan Africa (SSA), which experiences a disproportionately high cardiovascular disease (CVD) burden, population-based screening and prevention measures are hampered by low levels of knowledge about CVD and associated risk factors, and inaccurate perceptions of severity of risk. METHODS This protocol describes the planned processes for implementing community-driven participatory research, using a citizen science method to explore CVD risk perceptions and to develop community-specific advocacy and prevention strategies in the rural and urban SSA settings. Multi-disciplinary research teams in four selected African countries will engage with and train community members living in rural and urban communities as citizen scientists to facilitate conceptualization, co-designing of research, data gathering, and co-creation of knowledge that can lead to a shared agenda to support collaborative participation in community-engaged science. The emphasis is on robust community engagement, using mobile technology to support data gathering, participatory learning, and co-creation of knowledge and disease prevention advocacy. DISCUSSION Contextual processes applied and lessons learned in specific settings will support redefining or disassembling boundaries in participatory science to foster effective implementation of sustainable prevention intervention programmes in Low- and Middle-income countries.

['Okop|Kufre Joseph|KJ|http://orcid.org/0000-0002-0841-4588', 'Murphy|Kathy|K|', 'Lambert|Estelle Victoria|EV|', 'Kedir|Kiya|K|', 'Getachew|Hailemichael|H|', 'Howe|Rawleigh|R|', 'Niyibizi|Jean Berchmans|JB|', 'Ntawuyirushintege|Selemani|S|', 'Bavuma|Charlotte|C|', 'Rulisa|Stephen|S|', 'Kasenda|Stephen|S|', 'Chipeta|Effie|E|', 'Bunn|Christopher|C|', 'Crampin|Amelia C|AC|', 'Chapotera|Gertrude|G|', 'King|Abby C|AC|', 'Banchoff|Ann|A|', 'Winter|Sandra J|SJ|', 'Levitt|Naomi S|NS|']

2021

33768965

Prophylactic placement of inferior vena cava filters and the risk of death or venous thromboembolism in severe trauma patients: a retrospective study comparing two hospitals with different approaches.

Background Prophylactic use of inferior vena cava filters to prevent pulmonary embolism in trauma is controversial. The practice varies between hospitals and countries, in part due to conflicting evidence and guidelines. Purpose To compare the effects of pulmonary embolism, deep venous thrombosis and mortality in two hospitals using prophylactic inferior vena cava filter placement or prophylactic anticoagulation alone. Material and Methods Patients presenting with severe trauma were recruited from two level-1 trauma centres between January 2008 and December 2013. Recruited patients from an US hospital having prophylactic inferior vena cava filter inserted were compared to a Scandinavian hospital using prophylactic anticoagulation alone. Inclusion criteria were age >15 years, Injury Severity Score >15 and survival >24 h after hospital admission. Patients with venous thromboembolism diagnosed prior to inferior vena cava filter placement were excluded. A Cox proportional hazard regression model was used with adjustment for immortal time bias and predictor variables. Results In total, 951 patients were reviewed, 282 from an US hospital having inferior vena cava filters placed and 669 from a Scandinavian hospital without inferior vena cava filters. The mean age was 45.9 vs. 47.4 years and the mean Injury Severity Score was 29.8 vs. 25.9, respectively. Inferior vena cava filter placement was not associated with the hazard of pulmonary embolism (Hazard ratio=0.43; 95% confidence interval (CI) 0.12, 1.45; P=0.17) or mortality (Hazard ratio=1.16; 95% CI 0.70, 1.95; P=0.56). However, an increased rate of deep venous thrombosis was observed with inferior vena cava filters in place (Hazard ratio=3.75; 95% CI 1.68, 8.36; P=0.001). Conclusion In severely injured trauma patients, prophylactic inferior vena cava filter placement was not associated with pulmonary embolism or mortality. However, inferior vena cava filters were associated with increased rate of deep venous thrombosis.

['Trung Tran|Thien|T|https://orcid.org/0000-0001-5678-026X', 'Bjarnason|Haraldur|H|', 'McDonald|Jennifer|J|', 'Skaga|Nils Oddvar|NO|', 'Houghton|Damon E|DE|', 'Kim|Brian|B|', 'Stavem|Knut|K|', 'Kløw|Nils Einar|NE|https://orcid.org/0000-0002-6042-402X']

2021

34090053

In situ growth of copper-based metal-organic framework on a helical shape copper wire as a sorbent in stir-bar sorptive extraction of fenthion followed by corona discharge ion mobility spectrometry.

In this paper, a helical copper wire, coated with copper-benzene-1,4-dicarboxylic acid metal-organic framework (Cu-BDC) was used as a sorbent for stir-bar sorptive extraction of fenthion from water and fruit samples. The homogenous coating was fabricated through two simple and fast steps. The chemical conversion of copper substrate to copper hydroxide nanotubes (Cu(OH)2 NTs) was performed in an alkaline solution and then Cu-BDC was formed through a neutralization reaction. Corona discharge ion mobility spectrometry in positive mode was applied for the detection of fenthion. To improve the sensitivity of the method, some synthesis and extraction parameters affecting the extraction efficiency such as benzene-1,4-dicarboxylic acid concentration, ionic strength, sample pH, stirring rate, extraction temperature, and extraction time were investigated. The linear dynamic range between 0.5 and 80 μg L-1 and detection limit of 0.1 μg L-1 were obtained under optimal conditions. The intra- and inter-day relative standard deviations were less than 6.4 and 8.6%, respectively. The applicability of the method was examined for the analysis of different samples (i.e., well water, agricultural wastewater, and orange). The recovery for the determination of fenthion in spiked samples varied from 88 to 111%.

['Heidarbeigi|Maliheh|M|', 'Saraji|Mohammad|M|', 'Jafari|Mohammad Taghi|MT|']

2021

25444307

Detection of hospital outbreaks of influenza-like illness based on excess of incidence rates compared to the community.

The risk of nosocomial influenza-like illness (noso-ILI) compared with that of community-acquired ILI was calculated during 3 influenza seasons (2004-2007) at a 1100-bed university hospital with a total of 21,519 hospitalized patients. Outbreaks of noso-ILI occurred in each season, although a protective effect against noso-ILI was also identified for other wards.

['Vanhems|Philippe|P|', 'Voirin|Nicolas|N|', 'Bénet|Thomas|T|', 'Roche|Sylvain|S|', 'Escuret|Vanessa|V|', 'Régis|Corinne|C|', 'Giard|Marine|M|', 'Lina|Bruno|B|', 'Comte|Brigitte|B|', 'Coppéré|Brigitte|B|', 'Ecochard|René|R|']

2014

25046430

Production of tongue twisters by speakers with partial glossectomy.

Abstract A partial glossectomy can affect speech production. The goal of this study was to investigate the effect of the presence of a tumour as well as the glossectomy surgery on the patients' production of tongue twisters with the sounds [t] and [k]. Fifteen patients with tongue cancer and 10 healthy controls took part in the study. The outcome measures were the patients' speech acceptability, rate of errors, the time needed to produce the tongue twisters, pause duration between item repetitions and the tongue shape during the production of the consonants [t] and [k] before and after surgery. The patients' speech acceptability deteriorated after the surgery. Compared to controls, the patients' productions of the tongue twisters were slower but not more errorful. Following the surgery, their speed of production did not change, but the rate of errors was higher. Pause duration between items was longer in the patients than in the controls but did not increase from before to after surgery. Analysis of the patients' tongue shapes for the productions of [t] and [k] indicated a higher elevation following the surgery for the patients with flap reconstructions. The results demonstrated that the surgical resection of the tongue changed the error rate but not the speed of production for the patient. The differences in pause duration also indicate that the tumour and the surgical resection of the tongue may impact the phonological planning of the tongue twister.

['Bressmann|Tim|T|', 'Foltz|Anouschka|A|', 'Zimmermann|Jana|J|', 'Irish|Jonathan C|JC|']

2014

32501767

Melatonin attenuates diabetes-induced oxidative stress in spleen and suppression of splenocyte proliferation in laboratory mice.

Hyperglycaemic condition induced oxidative stress in diabetic individuals caused oxidative damages of internal organs, including immune organ spleen. We studied the effects of low doses of melatonin (25, 50, and 100 µg/100g. B.wt./day) on histoarchitecture, oxidative stress, and splenocyte proliferation in streptozotocin-induced diabetic mice. Melatonin significantly resisted the increase in blood glucose levels and showed a dose-dependent effect on circulatory melatonin, body weight, and relative spleen weight in diabetic mice. Exogenous melatonin suppressed the diabetes-induced lipid peroxidation and increased the activity of the antioxidant enzymes and antioxidant GSH in the spleen tissue of diabetic mice in a dose-dependent manner. Melatonin improved the reactivity of Nrf-2 and HO-1 in the spleen of diabetic mice. Melatonin treatment normalised the splenic cellularity and increased the splenocyte proliferation in a dose-dependent manner. The present study may suggest the dose-dependent effect of melatonin in attenuation of oxidative stress and suppression of splenocyte proliferation in diabetic mice.

['Sutradhar|Sangita|S|', 'Deb|Anindita|A|', 'Singh|Shiv Shankar|SS|http://orcid.org/0000-0002-1053-1580']

2020

33261954

Integrated metabolomics and network pharmacology approach to reveal immunomodulatory mechanisms of Yupingfeng granules.

Systems biology is an approach that employs modern biological techniques and methods to combine the overall regulation of the body by Chinese herbal formulas with systems analysis at the molecular level. In this study, the underlying immunomodulatory mechanisms of yupingfeng granules (YPFG) were investigated based on the integration of metabolomics and network pharmacology methods. Selected routine peripheral blood indicators, body weight, and organ indices related to immunity were firstly measured in order to evaluate the effects of YPFG in cyclophosphamide-induced immunocompromised rats. Plasma metabolomics analyses were carried out by UPLC-Q-TOF-MS combined with a multivariate data analysis. Our study indicates that the potential regulatory mechanism was related to bile acid and glycerophospholipid metabolism, involving the regulation of 11 metabolites, including 8 bile acids, 1 phosphatidylserine, and 2 phosphatidylethanolamines. By means of network pharmacology, the compound-target network between potential active components of YPFG and immune dysregulation was constructed, which releated to estrogen receptor, PPAR, MAPK, PI3K-Akt, JNK signaling pathways, and ubiquitin-mediated protein degradation. The immunomodulatory effect of YPFG may be exerted through regulating lipid metabolism, then bile acid metabolism and inflammation were affected. Biological verification was also performed on cyclophosphamide-induced immunocompromised BALB/c mice. Flavonoid and saponin, two types of compounds in YPFG, were found to be the major active ingredients in the immunomodulatory effects of YPFG, and these components may regulate the abnormal metabolism of bile acids by enhancing the expression of FXR and LXRα. This work elucidated active ingredients, potential biomarkers, and mechanisms of action in the immunoregulatory effects of YPFG from the perspective of systems biology, which provides a scientific basis for its precise clinical medication.

['Li|Moying|M|', 'Gao|Yongjian|Y|', 'Yue|Xinyi|X|', 'Zhang|Bei|B|', 'Zhou|Haifeng|H|', 'Yuan|Chunping|C|', 'Wu|Tong|T|']

2021

33530943

Development of an explicit tool assessing potentially inappropriate medication use in Hong Kong elder patients.

BACKGROUND Potentially inappropriate medication (PIM) use has adverse effects on health, particularly in elder patients. Various country-specific explicit criteria have been developed to measure the appropriateness of prescribing worldwide. However, it is difficult to apply the criteria developed from other regions to measure and guide the local prescribing practice in Hong Kong. This study aims to develop a Hong Kong-specific PIM assessing tool from previously published criteria and validate this tool using the modified Delphi method. METHODS A disease-oriented Hong Kong-specific preliminary PIM list was developed based on nine sets of reference criteria selected from a literature review. Any medication or medication class appeared in at least two sets of the reference criteria as well as its related medical conditions were selected as PIM candidates. After examining the availability of PIM candidates by the Hong Kong Hospital Authority drug formulary, the Hong Kong-specific preliminary PIM list was validated by a two-round of modified Delphi process. Eight experts from different specialties were invited to rate the degree of inappropriateness of each PIM candidate using a five-point Likert scale. The experts were also encouraged to propose therapeutic alternatives and new PIM candidates not covered by the preliminary PIM list. The PIM candidates that the expert panel didn't reach consensus on were excluded from the final Hong Kong-specific PIM list. RESULTS After two rounds of the Delphi process, eight PIM candidates remained questionable and thus were excluded from the PIM list. The final Hong Kong-specific PIM list included a total of 164 statements applicable to older adults aged 65 years or above, among which 77 were under PIMs independent of diagnoses, and 87 were under PIMs considering specific medical conditions. CONCLUSIONS The Hong Kong-specific PIM list can be used as a quality measure and an educational tool to improve the local prescribing quality. Further studies should validate its association with adverse health outcomes in clinical and research settings.

['Zhang|Huanyu|H|', 'Wong|Eliza Ly|EL|0000-0001-9983-6219', 'Yeoh|Eng-Kiong|EK|', 'Ma|Bosco Hm|BH|']

2021

34453580

Contemporary treatment trends for upper urinary tract stones in a total population analysis in Germany from 2006 to 2019: will shock wave lithotripsy become extinct?

PURPOSE To describe the change in upper urinary tract stone management in Germany over a 14-year period. METHODS Using remote data processing we analyzed the nationwide German billing data from 2006 to 2019. To analyze the clinics' case numbers and regional trends, we used the reimbursement.INFO tool based on standardized quality reports of all German hospitals. To also cover shock wave lithotripsy (SWL) as an outpatient procedure, we analyzed the research database of the Institute for Applied Health Research with a representative anonymous sample of 4 million insured persons. RESULTS The number of inpatient interventional therapies for upper tract urolithiasis in Germany increased from 70,099 cases in 2006 to 94,815 cases in 2019 (trend p < 0.0001). In-hospital SWL declined from 41,687 cases in 2006 to 10,724 cases in 2019 (decline of 74%; trend p < 0.0001). The percentage of SWL as an outpatient procedure increased between 2013 and 2018 from 36 to 46% of all performed SWL, while total SWL case numbers declined. Contrarily, the number of ureteroscopies increased from 32,203 cases in 2006 to 78,125 cases in 2019 (increase of 143%; trend p < 0.0001). The number of percutaneous nephrolithotomy also increased from 1673 cases in 2006 to 8937 in 2019 (increase of 434%; trend p < 0.0001). CONCLUSION We observed an increase in interventional therapy for upper tract urolithiasis in Germany with a dramatic shift from SWL to endoscopic/percutaneous treatment. These changes may be attributed to enormous technological advances of the endoscopic armamentarium and to reimbursement issues.

['Herout|Roman|R|http://orcid.org/0000-0002-9634-2632', 'Baunacke|Martin|M|', 'Groeben|Christer|C|', 'Aksoy|Cem|C|', 'Volkmer|Björn|B|', 'Schmidt|Marcel|M|', 'Eisenmenger|Nicole|N|', 'Koch|Rainer|R|', 'Oehlschläger|Sven|S|', 'Thomas|Christian|C|', 'Huber|Johannes|J|']

2021

32944042

Thiopurine S-methyltransferase and Pemphigus Vulgaris: A Phenotype-Genotype Study.

Background & Objective Thiopurine drugs are considered as a treatment modality in various autoimmune disorders including pemphigus vulgaris (PV). These drugs are metabolized by an enzyme "Thiopurine S-methyl transferase" (TPMT). Various variants of this enzyme may have decreased activity leading to serious drug side effects. To investigate the phenotype and genotype of TPMT in PV patients receiving thiopurine drugs. Methods A total of 50 patients (29 women and 21 men) with pemphigus vulgaris treating with standard dose of Thiopurine drugs were selected. Sex, age, result of liver function test and complete blood count were recorded. Genotyping of two common non-functional allele (TPMT*2 and TPMT*3C) by Allele-specific and RFLP-PCR was performed. TPMT enzymatic level was determined by an ELISA based method. Results Of patients, 36 (72%) were found to have normal TPMT level; and 12, (24%) had higher level of enzyme and 2, 4% had low TPMT enzyme, but none of the patients showed mutant TPMT*2 and TPMT*3C alleles. None of the patients showed hepatotoxicity and bone marrow suppression. Conclusion The phenotypic assay based on ELISA method may have false positive and misleading results but genotyping using PCR-RFLP and allele specific PCR is accurate, simple and cost-effective and can be used in patients decided to undergo thiopurine treatment.

['Mokhtari|Maral|M|', 'Mostanbet|Farzaneh|F|', 'Nekooee Fard|Saideh|S|', 'Shekarkhar|Golsa|G|', 'Sepaskhah|Mozhdeh|M|', 'Sadati|Maryam Sadat|MS|']

2020

31812956

Interaction of Blood Pressure Lowering and Alteplase Dose in Acute Ischemic Stroke: Results of the Enhanced Control of Hypertension and Thrombolysis Stroke Study.

OBJECTIVE To determine the extent to which the effects of intensive blood pressure (BP) lowering are modified by doses of alteplase in thrombolysis-eligible acute ischemic stroke (AIS) patients. METHODS Prespecified analyses of the Enhanced Control of Hypertension and Thrombolysis Stroke Study for patients enrolled in both arms: (i) low-dose (0.6 mg/kg body weight) or standard-dose (0.9 mg/kg) alteplase and (ii) intensive (target systolic BP [SBP] 130-140 mm Hg) or guideline-recommended (target SBP <180 mm Hg) BP management. The primary outcome was functional recovery, measured by a shift in scores on modified Rankin scale at 90 days. The safety outcome was any intracranial hemorrhage (ICH). RESULTS There were 925 participants (mean age 67 years, 39% female, 77% Asian) randomized to both arms: 242 randomly assigned to guideline/standard-dose (GS); 234 to guideline/low-dose (GL); 227 to intensive/standard-dose (IS); and 222 to intensive/low-dose (IL). Overall, average SBP levels within 24 h were lower in the low-dose compared to standard-dose alteplase group (146 and 144 vs. 151 and 150 mm Hg, for GS and GL vs. IS and IL, respectively, p < 0.0001). There was no heterogeneity of the effects of BP lowering (intensive vs. guideline) on functional recovery between standard-dose (OR 0.81, 95% CI 0.59-1.12) and low-dose alteplase (1.06, 0.77-1.47; p = 0.25 for interaction). Similar results were observed for ICH (p = 0.50 for interaction). CONCLUSIONS In thrombolysis-treated patients with predominantly mild-to-moderate severity AIS, intensive BP lowering neither improve functional recovery, either with low- or standard-dose intravenous alteplase, nor beneficially interact with low-dose alteplase in reducing ICH. TRIAL REGISTRATION The trial is registered with ClinicalTrials.gov (NCT01422616).

['Wang|Xia|X|', 'Song|Lili|L|', 'Yang|Jie|J|', 'Sun|Lingli|L|', 'Moullaali|Tom J|TJ|', 'Sandset|Else Charlotte|EC|', 'Delcourt|Candice|C|', 'Lindley|Richard I|RI|', 'Robinson|Thompson G|TG|', 'Minhas|Jatinder S|JS|', 'Arima|Hisatomi|H|', 'Chalmers|John|J|', 'Kim|Jong S|JS|', 'Sharma|Vijay|V|', 'Wang|Ji-Guang|JG|', 'Pontes-Neto|Octávio|O|', 'Lavados|Pablo M|PM|', 'Olavarría|Verónica V|VV|', 'Lee|Tsong-Hai|TH|', 'Levi|Christopher|C|', 'Martins|Sheila O|SO|', 'Thang|Nguyen H|NH|', 'Anderson|Craig S|CS|', '|||']

2019

26648933

Investigating the Antigen Specificity of Multiple Sclerosis Central Nervous System-Derived Immunoglobulins.

The central nervous system (CNS) of patients with multiple sclerosis (MS) is the site where disease pathology is evident. Damaged CNS tissue is commonly associated with immune cell infiltration. This infiltrate often includes B cells that are found in multiple locations throughout the CNS, including the cerebrospinal fluid (CSF), parenchyma, and the meninges, frequently forming tertiary lymphoid structures in the latter. Several groups, including our own, have shown that B cells from distinct locations within the MS CNS are clonally related and display the characteristics of an antigen-driven response. However, the antigen(s) driving this response have yet to be conclusively defined. To explore the antigen specificity of the MS B cell response, we produced recombinant human immunoglobulin (rIgG) from a series of expanded B cell clones that we isolated from the CNS tissue of six MS brains. The specificity of these MS-derived rIgG and control rIgG derived from non-MS tissues was then examined using multiple methodologies that included testing individual candidate antigens, screening with high-throughput antigen arrays and evaluating binding to CNS-derived cell lines. We report that while several MS-derived rIgG recognized particular antigens, including neurofilament light and a protocadherin isoform, none were unique to MS, as non-MS-derived rIgG used as controls invariably displayed similar binding specificities. We conclude that while MS CNS resident B cells display the characteristics of an antigen-driven B cell response, the antigen(s) driving this response remain at large.

['Willis|Simon N|SN|', 'Stathopoulos|Panos|P|', 'Chastre|Anne|A|', 'Compton|Shannon D|SD|', 'Hafler|David A|DA|', "O'Connor|Kevin C|KC|"]

2015

32886425

Assessment of provider perspectives on otoprotection research for children and adolescents: A Children's Oncology Group Cancer Control and Supportive Care Committee survey.

BACKGROUND Cisplatin-induced hearing loss (CIHL) is a common and debilitating toxicity for childhood cancer survivors. Understanding provider perspectives is crucial to developing otoprotection studies that are both informative and feasible. Two international trials (ACCL0431 and SIOPEL6) investigated the drug sodium thiosulfate (STS) as an otoprotectant, but definitive interpretation of the findings of these trials has been challenging. Adoption of STS has therefore been uneven, and provider perspectives on its role are unknown. PROCEDURE The Children's Oncology Group (COG) Cancer Control and Supportive Care Neurotoxicity Subcommittee therefore conducted a survey of providers at COG institutions to determine perspectives on pediatric otoprotection practices and research surrounding three major themes: (1) prevalence of routine use of STS with cisplatin-based regimens, (2) application of audiometry to cisplatin therapy, and (3) preferred modalities for otoprotection research. RESULTS Survey respondents (45%, 44/98 surveyed institutions) were of diverse institutional sizes, practice settings, and geographical locations primarily in the United States and Canada. Overall, respondents considered CIHL an important toxicity and indicated strong enthusiasm for future studies (98%, 40/41). Results indicated that while STS was the current or planned standard of care in a minority of responding institutions (36%, 16/44), most sites were receptive to its inclusion in appropriate study designs. Application of audiometry for ototoxicity monitoring varied widely across sites. For otoprotection research, systemic agents were preferred (68%, 28/41) as compared with intratympanic approaches. CONCLUSION These results suggest that pediatric otoprotection trials remain of interest to providers; the emphasis of these trials should remain on systemic and not intratympanic therapy.

['Orgel|Etan|E|0000-0002-1487-6818', 'Freyer|David R|DR|0000-0001-5280-990X', 'Ullrich|Nicole J|NJ|', 'Hardy|Kristina K|KK|', 'Thomas|Stefanie M|SM|', 'Dvorak|Christopher C|CC|0000-0002-6146-3952', 'Esbenshade|Adam J|AJ|']

2020

23438149

Salt (sodium chloride) content of retail samples of Nigerian white bread: implications for the daily salt intake of normotensive and hypertensive adults.

BACKGROUND Bread has been identified as a major contributor to the excessive salt (sodium chloride) intake of consumers in many countries, some of which have very high incidences of hypertension and related cardiovascular complications, such as stroke. This has prompted a global rise in interest in the salt content of breads produced and consumed in many other countries. METHODS The sodium contents of retail samples of 100 brands of Nigerian white bread were determined by photometry with a view to estimating the relative contribution of bread to the recommended daily sodium intake of both normotensive and hypertensive adults in the country. RESULTS The salt content of the bread samples varied extensively, ranging from 0.51 g per 100 g (0.51%) to 1.8 g per 100 g (1.8%). The average salt content was 1.36 g per 100 g. Based on an estimated consumption of six slices of bread (about 180 g) per meal of bread, this equates to a daily intake of between 0.99 g and 3.33 g of salt from bread alone. This represents between 19.8% and 66.6% of the recommended daily allowance of 5 g for normotensive adults, and between 24.75% and 83.25% of the recommended daily allowance of 4 g for hypertensive adults. CONCLUSIONS The consumption of some brands of bread by normotensive and hypertensive adults puts them at great risk of exceeding their recommended daily allowance for salt. Thus, there is an urgent need to regulate the amount of salt added to bread. In the interim, compelling bakers to declare the salt content of their products on the packaging could help consumers, especially hypertensive adults, avoid brands with a high salt content.

['Nwanguma|B C|BC|', 'Okorie|C H|CH|']

2013

34127004

De novo design of a pH-triggered self-assembled β-hairpin nanopeptide with the dual biological functions for antibacterial and entrapment.

BACKGROUND Acid-tolerant enteric pathogens can evade small intestinal acid barriers, colonize and infect the intestinal tract. However, broad-spectrum antibiotics are not the best therapeutic strategy because of the disruption of intestinal flora caused by its indiscriminate antimicrobial activity against beneficial and harmful bacteria. So that is what inspired us to combine pH regulation with nanotechnology to develop a pH-triggered site-targeted antimicrobial peptide with entrapping function. RESULTS A pH-triggered dual biological functional self-assembled peptide (SAP) was designed according to the features of amino-acid building blocks and the diagonal cation-π interaction principle. The results of characterization experiments showed that changes in pH conditions could trigger microstructural transformation of the nanopeptide from nanospheres to nanofibers. The subsequent antibacterial and toxicity experiments determined that SAP had great antimicrobial activity against Escherichia coli, Salmonella typhimurium, Listeria monocytogenes, and Bacillus cereus above 15.6 μg/mL under acidic conditions by disrupting bacterial membrane integrity, excellent biocompatibility in vitro even at 250 μg/mL and high tolerance in physical environment. Moreover, at peptide concentrations greater than 62.5 μg/mL, SAP showed the entrapment property, which played an important role in phagocytic clearance in infection forces. Meanwhile, the in vivo results revealed that SAP possessed excellent therapeutic effect and good biosafety. CONCLUSIONS Our study revealed the antibacterial activity of a short β-hairpin forming self-assembled peptide, and established an innovative design strategy for peptide-based nanomaterials and a new treatment strategy for gastrointestinal bacterial infections.

['Li|Qiuke|Q|', 'Li|Jinze|J|', 'Yu|Weikang|W|', 'Wang|Zhihua|Z|', 'Li|Jiawei|J|', 'Feng|Xingjun|X|', 'Wang|Jiajun|J|', 'Shan|Anshan|A|']

2021

24698197

The energetic and nutritional yields from insectivory for Kasekela chimpanzees.

Insectivory is hypothesized to be an important source of macronutrients, minerals, and vitamins for chimpanzees (Pan troglodytes), yet nutritional data based on actual intake are lacking. Drawing on observations from 2008 to 2010 and recently published nutritional assays, we determined the energy, macronutrient and mineral yields for termite-fishing (Macrotermes), ant-dipping (Dorylus), and ant-fishing (Camponotus) by the Kasekela chimpanzees of Gombe National Park, Tanzania. We also estimated the yields from consumption of weaver ants (Oecophylla) and termite alates (Macrotermes and Pseudacanthotermes). On days when chimpanzees were observed to prey on insects, the time spent in insectivorous behavior ranged from <1 min to over 4 h. After excluding partial bouts and those of <1 min duration, ant-dipping bouts were of significantly shorter duration than the other two forms of tool-assisted insectivory but provided the highest mass intake rate. Termite-fishing bouts were of significantly longer duration than ant-dipping and had a lower mass intake rate, but provided higher mean and maximum mass yields. Ant-fishing bouts were comparable to termite-fishing bouts in duration but had significantly lower mass intake rates. Mean and maximum all-day yields from termite-fishing and ant-dipping contributed to or met estimated recommended intake (ERI) values for a broad array of minerals. The mean and maximum all-day yields of other insects consistently contributed to the ERI only for manganese. All forms of insectivory provided small but probably non-trivial amounts of fat and protein. We conclude that different forms of insectivory have the potential to address different nutritional needs for Kasekela chimpanzees. Other than honeybees, insects have received little attention as potential foods for hominins. Our results suggest that ants and (on a seasonal basis) termites would have been viable sources of fat, high-quality protein and minerals for extinct hominins employing Pan-like subsistence technology in East African woodlands.

["O'Malley|Robert C|RC|", 'Power|Michael L|ML|']

2014

27604750

Glycogen synthase kinase 3 (GSK3)-inhibitor SB216763 promotes the conversion of human umbilical cord mesenchymal stem cells into neural precursors in adherent culture.

The ability to generate neural progenitor cells from human umbilical cord mesenchymal stem cells (hUC-MSCs) has provided an option to treat neurodegenerative diseases. To establish a method for this purpose, we characterized the early neural markers of hUC-MSCs-derived cells under different conditions. We found that neither the elimination of signals for alternative fate nor N2 supplement was sufficient to differentiate hUC-MSCs into neural precursor cells, but the GSK3 inhibitor SB216763 could promote an efficient neural commitment of hUC-MSCs. The results indicated that Wnt/β-catenin might play an important role during the early neural differentiation of hUC-MSCs. Here, we report a method for hUC-MSCs to commit efficiently into a neural fate within a short period of time. This protocol provides an efficient method for hUC-MSCs-based neural regeneration.

['Gao|Liyang|L|', 'Zhao|Mingyan|M|', 'Li|Peng|P|', 'Kong|Junchao|J|', 'Liu|Zhijun|Z|', 'Chen|Yonghua|Y|', 'Huang|Rui|R|', 'Chu|Jiaqi|J|', 'Quan|Juanhua|J|', 'Zeng|Rong|R|']

2017

34837184

An Ultracompact Real-Time Fluorescence Loop-Mediated Isothermal Amplification (LAMP) Analyzer.

Low-cost access to the highly sensitive and specific detection of the pathogen in the field is a crucial attribute for the next generation point-of-care (POC) platforms. In this work, we developed a real-time fluorescence nucleic acid testing device with automated and scalable sample preparation capability for field malaria diagnosis. The palm-sized battery-powered analyzer equipped with a disposable microfluidic reagent compact disc described in the companion Chap. 16 which facilitates four isothermal nucleic acid tests in parallel from raw blood samples to answer. The platform has a user-friendly interface such as touchscreen LCD and smartphone data connectivity for on-site and remote healthcare delivery, respectively. The chapter mainly focuses on describing integration procedures of the real-time fluorescence LAMP analyzer and the validation of its subsystems. The device cost is significantly reduced compared to the commercial benchtop real-time machine and other existing POC platforms. As a platform technology, self-sustainable, portable, low-cost, and easy-to-use analyzer design should create a new paradigm of molecular diagnosis toward a variety of infectious diseases at the point of need.

['Choi|Gihoon|G|', 'Guan|Weihua|W|']

2022

34296205

A native cell membrane nanoparticles system allows for high-quality functional proteoliposome reconstitution.

Proteoliposomes mimic the cell membrane environment allowing for structural and functional membrane protein analyses as well as antigen presenting and drug delivery devices. To make proteoliposomes, purified functional membrane proteins are required. Detergents have traditionally been used for the first step in this process However, they can irreversibly denature or render membrane proteins unstable, and the necessary removal of detergents after reconstitution can decrease proteoliposome yields. The recently developed native cell membrane nanoparticles (NCMN) system has provided a variety of detergent-free alternatives for membrane protein preparation for structural biology research. Here we attempt to employ the MCMN system for the functional reconstitution of channels into proteoliposomes. NCMN polymers NCMNP1-1 and NCMNP7-1, members of a NCMN polymer library that have been successful in extraction and affinity purification of a number of intrinsic membrane proteins, were selected for the purification and subsequent reconstitution of three bacterial channels: KcsA and the mechanosensitive channels of large and small conductance (MscL and MscS). We found that channels in NCMN particles, which appeared to be remarkably stable when stored at 4 °C, can be reconstituted into bilayers by simply incubating with lipids. We show that the resulting proteoliposomes can be patched for electrophysiological studies or used for the generation of liposome-based nanodevices. In sum, the findings demonstrate that the NCMN system is a simple and robust membrane protein extraction and reconstitution approach for making high-quality functional proteoliposomes that could significantly impact membrane protein research and the development of nanodevices.

['Yang|Limin|L|', 'Catalano|Claudio|C|', 'Xu|Yunyao|Y|', 'Qiu|Weihua|W|', 'Zhang|Dongyu|D|', 'McDermott|Ann|A|', 'Guo|Youzhong|Y|', 'Blount|Paul|P|']

2021

24734859

Movement therapies for the self-management of chronic pain symptoms.

OBJECTIVES Chronic pain management typically consists of prescription medications or provider-based, behavioral, or interventional procedures which are often ineffective, may be costly, and can be associated with undesirable side effects. Because chronic pain affects the whole person (body, mind, and spirit), patient-centered complementary and integrative medicine (CIM) therapies that acknowledge the patients' roles in their own healing processes have the potential to provide more efficient and comprehensive chronic pain management. Active self-care CIM therapies (ACT-CIM) allow for a more diverse, patient-centered treatment of complex symptoms, promote self-management, and are relatively safe and cost-effective. To date, there are no systematic reviews examining the full range of ACT-CIM used for chronic pain symptom management. METHODS A systematic review was conducted, using Samueli Institute's rapid evidence assessment of the literature methodology, to rigorously assess both the quality of the research on ACT-CIM modalities and the evidence for their efficacy and effectiveness in treating chronic pain symptoms. A working group of subject matter experts was also convened to evaluate the overall literature pool and develop recommendations for the use and implementation of these modalities. RESULTS Following key database searches, 146 randomized controlled trials were included in the review, 30 of which investigated movement therapies, as defined by the authors. CONCLUSIONS This article summarizes the current evidence, quality, efficacy, and safety of these modalities. Recommendations and next steps to move this field of research forward are also discussed. The entire scope of the review is detailed throughout the current Pain Medicine supplement.

['Lee|Courtney|C|', 'Crawford|Cindy|C|', 'Schoomaker|Eric|E|', '|||']

2014

30576692

Effect of density-dependent individual movement on emerging spatial population distribution: Brownian motion vs Levy flights.

Individual animal movement has been a focus of intense research and considerable controversy over the last two decades, however the understanding of wider ecological implications of various movement behaviours is lacking. In this paper, we consider this issue in the context of pattern formation. Using an individual-based modelling approach and computer simulations, we first show that density dependence ("auto-taxis") of the individual movement in a population of random walkers typically results in the formation of a strongly heterogeneous population distribution consisting of clearly defined animal clusters or patches. We then show that, when the movement takes place in a large spatial domain, the properties of the clusters are significantly different in the populations of Brownian and non-Brownian walkers. Whilst clusters tend to be stable in the case of Brownian motion, in the population of Levy walkers clusters are dynamical so that the number of clusters fluctuates in the course of time. We also show that the population dynamics of non-Brownian walkers exhibits two different time scales: a short time scale of the relaxation of the initial condition and a long time scale when one type of dynamics is replaced by another. Finally, we show that the distribution of sample values in the populations of Brownian and non-Brownian walkers is significantly different.

['Ellis|John|J|', 'Petrovskaya|Natalia|N|', 'Petrovskii|Sergei|S|']

2019

28379687

Kinetic Requirements for Spatiotemporal Chemical Imaging with Fluorescent Nanosensors.

Fluorescent nanosensors are powerful tools for basic research and bioanalytical applications. Individual nanosensors are able to detect single molecules, while ensembles of nanosensors can be used to measure the bulk concentration of an analyte. Collective imaging of multiple nanosensors could provide both spatial and temporal chemical information from the nano- to the microscale. This type of chemical imaging with nanosensors would be very attractive to study processes such as chemical signaling between cells (e.g., neurons). So far, it is not understood what processes are resolvable (concentration, time, space) and how optimal sensors should be designed. Here, we develop a theoretical framework to simulate the fluorescence image of arrays of nanosensors in response to a concentration gradient. For that purpose, binding and unbinding of the analyte is simulated for each single nanosensor by using a Monte Carlo simulation and varying rate constants (kon, koff). Multiple nanosensors are arranged on a surface and exposed to a concentration pattern cA(x,y,t) of an analyte. We account for the resolution limit of light microscopy (Abbe limit) and the acquisition speed and resolution of optical setups and determine the resulting response images ΔI(x,y,t). Consequently, we introduce terms for the spatial and temporal resolution and simulate phase diagrams for different rate constants that allow us to predict how a sensor should be designed to provide a desired spatial and temporal resolution. Our results show, for example, that imaging of neurotransmitter release requires rate constants of kon = 106 M-1 s-1and koff = 102 s-1 in many scenarios, which corresponds to high dissociation constants of Kd > 100 μM. This work predicts if a given fluorescent nanosensor array (rate constants, size, shape, geometry, density) is able to resolve fast concentration changes such as neurotransmitter release from cells. Additionally, we provide rational design principles to engineer nanosensors for chemical imaging.

['Meyer|Daniel|D|', 'Hagemann|Annika|A|', 'Kruss|Sebastian|S|0000-0003-0638-9822']

2017

28472292

Calcium-dependent protein kinase OsCPK10 mediates both drought tolerance and blast disease resistance in rice plants.

Plant growth and productivity is negatively affected by different stresses. Most stresses trigger calcium signals that initiate acclimation responses in plants. The multigene family of plant calcium-dependent protein kinases (CPKs) functions in multiple stress responses by transducing calcium signals into phosphorylation events. This work reports that the OsCPK10 isoform positively mediates tolerance to different stresses in rice plants by enhancing their antioxidant capacity and protecting them from reactive oxygen species (ROS) damage, with the uncontrolled generation of ROS being a common feature of these stresses. Here, we show that the constitutive accumulation of an HA-tagged OsCPK10 full-length protein enhances the hydrogen peroxide detoxifying capacity of rice plants during desiccation. This is achived by modulating the accumulation of catalase proteins, which reduces the extent of lipid peroxidation and protects the integrity of cell membranes, resulting in drought tolerance. OsCPK10HA accumulation also confers blast disease resistance by interfering with fungal necrotrophic growth via a reduction in the accumulation of hydrogen peroxide. Furthermore, we show by bimolecular complementation assays that OsCPK10 is a plasma membrane protein that physically interacts in vivo with catalase A. OsCPK10 therefore appears to be a good molecular target to improve tolerance to abiotic stresses as well as to blast disease, which limit rice crop productivity.

['Bundó|Mireia|M|', 'Coca|María|M|']

2017

26300545

Evaluation of alginate-whey protein microcapsules for intestinal delivery of lipophilic compounds in pigs.

BACKGROUND In animal care and management, there is an increasing demand for convenient methods of oral delivery of bioactive compounds to specific segments of an animal's gastrointestinal tract. The objective of this study was to test the suitability of microcapsules made with alginate and whey proteins of two different sizes (250 and 800 µm; containing 72 and 76 g kg(-1) of carvacrol respectively) for intestinal delivery of carvacrol in pigs. RESULTS Encapsulated carvacrol was completely released from the microcapsules after 5 h incubation in simulated intestinal fluids or 6 h in (ex vivo) ileal digesta, whereas release in simulated gastric fluid was minimal. Tests with growing pigs showed over 95% of unencapsulated carvacrol was absorbed or metabolized in the stomach and the duodenum. Encapsulation effectively minimized carvacrol absorption in the stomach (P < 0.05), and increased carvacrol recovery in the small intestine (P < 0.05). Encapsulated carvacrol was completely released from both small and large size capsules within the gastrointestinal tract of pigs. Larger size microcapsules showed a slower in vitro release and greater in vivo recovery of carvacrol in the small intestine (P < 0.05) than the smaller ones. CONCLUSION This study indicates alginate-whey protein microencapsulation is a feasible approach for targeted oral delivery of hydrophobic compounds to pig intestines; increasing capsule size increased delivery of carvacrol to the end of the small intestine. © 2015 Her Majesty the Queen in Right of Canada Journal of the Science of Food and Agriculture © 2015 Society of Chemical Industry.

['Zhang|Yonggang|Y|', 'Wang|Qi C|QC|', 'Yu|Hai|H|', 'Zhu|Julia|J|', 'de Lange|Kees|K|', 'Yin|Yulong|Y|', 'Wang|Qi|Q|', 'Gong|Joshua|J|']

2016

25160130

Dexmedetomidine utilisation and outcomes of children with trisomy 21 undergoing congenital heart disease surgery.

INTRODUCTION The diagnosis of trisomy 21 in children has been associated with failed extubation after CHD surgery. Dexmedetomidine may be a useful agent to improve postoperative outcomes in these patients, such as ventilator time, ICU length of stay, or hospital length of stay. MATERIALS AND METHODS The Pediatric Health Information System database was queried from January, 2008 to December, 2010 for patients with trisomy 21 who underwent CHD surgery. Patients who received dexmedetomidine were matched to patients who did not by propensity score. The primary outcome was ventilator days charged, and secondary outcomes included ICU and hospital length of stay. RESULTS A total of 1088 patients (544 matched pairs) met inclusion criteria. Patient characteristics were similar, with the exception of more patients in the dexmedetomidine group undergoing repair of complete atrioventricular canal and fewer undergoing mechanical valve replacement (p<0.01). More patients in the dexmedetomidine group were administered milrinone, epinephrine, vasopressin, benzodiazepines, opiates, and adjunct pain and sedative medications (p<0.01). The dexmedetomidine group had greater time on the ventilator [7 (4.5-11) versus 6 (4-10) days (median, interquartile range) p<0.01] and similar ICU length of stay, hospital length of stay, and mortality compared with controls. Mixed-effects modelling clustered on institution did not show beneficial effect of dexmedetomidine on ventilator time, ICU stay, or hospital length of stay. CONCLUSIONS The use of dexmedetomidine was not associated with the decreased ventilatory time. Routine use of dexmedetomidine is not warranted in this patient population.

['Moffett|Brady S|BS|', 'Mossad|Emad B|EB|', 'Tobias|Joseph D|JD|', 'Cabrera|Antonio G|AG|']

2015

30448723

Novel dauricine derivatives suppress cancer via autophagy-dependent cell death.

Eleven dauricine derivatives were synthesized and evaluated for their anti-cancer effect in different cancer cells and their autophagic activity in HeLa model cell. Among these newly synthesized compounds, carbamates 2a, 2b, carbonyl ester 3a and sulfonyl ester 4a exhibited potent cytotoxic effects on tested cancer cells with IC50 values ranged from 2.72 to 12.53 μM, which were more potent than that of dauricine (higher than 15.53 μM). The above four derivatives are validated to induce autophagy-dependent cell death in HeLa cancer cells. These findings offer us a promising source for generating novel autophagic enhancers for anti-cancer therapy.

['Zhou|Xiaobo|X|', 'Qu|Yuan Qing|YQ|', 'Zheng|Zhiyuan|Z|', 'Law|Betty Yuen Kwan|BYK|', 'Mok|Simon Wing Fai|SWF|', 'Jiang|Zhi-Hong|ZH|', 'Wong|Vincent Kam Wai|VKW|', 'Bai|Li-Ping|LP|']

2019

32381401

Knowledge and Compliance of Dentists' and Dental Students' With Respect to Relevant Guidelines for Prescribing Antibiotic Prophylaxis for the Prevention of Infective Endocarditis: A Systematic Review.

BACKGROUND It is essential that dental clinicians, including dental students, have sufficient knowledge of and adhere to relevant prophylactic guidelines for the prevention of infective endocarditis. Because the overprescription of antibiotics contributes to the development of drug resistance, antibiotic stewardship should be at the forefront of patient care. The aim of this review is to determine the level of knowledge and compliance of dentists and dental students to relevant guidelines regarding antibiotic prophylaxis for the prevention of infective endocarditis. In addition, we aim to identify the factors that influence the level of knowledge and compliance. MATERIALS AND METHODS The following electronic databases were searched: MEDLINE (OvidSP), Scopus, Web of Science, CINAHL (EBSCOhost), and Informit Health Database. Inclusion criteria were (1) published quantitative studies that assessed dentists' and dental students' knowledge of and compliance with relevant guidelines for prescribing antibiotic prophylaxis for the prevention of infective endocarditis; (2) studies in English; and (3) studies published on any date. The National Institutes of Health, National Heart, Lung and Blood Institute Quality Assessment Tool for Observational Cohort and Cross-Sectional Studies was used to appraise each of the included studies. The required data were collected and formatted to provide a narrative synthesis. RESULTS A total of 3427 articles were identified, 167 full-text articles were reviewed, and 43 articles from 37 studies were included. Overall knowledge of guidelines ranged from as low as 1.9% to as high as 100% depending on the detail being examined. No statistically significant difference was found between dental students' and qualified dentists' knowledge. Overall compliance with guidelines ranged from 40% to 81%. Respondents' age and postgraduate training were the variables most associated with dentists' knowledge of guidelines for infective endocarditis. A limitation of this review is that only quantitative studies in English were assessed. CONCLUSION Knowledge levels of guidelines for antibiotic prophylaxis varied greatly depending on the study design, respondent characteristics, and time period factors. There is a lack of research published on compliance rates for dentists and dental students with respect to guidelines for the prevention of infective endocarditis.

['Cummins|Jessie|J|', 'McCarthy|Michael|M|', 'Esterman|Adrian|A|', 'Karve|Avanti|A|', 'Lee|Andrew|A|']

2020

29411965

Hollow Pt-Functionalized SnO2 Hemipill Network Formation Using a Bacterial Skeleton for the Noninvasive Diagnosis of Diabetes.

Hollow-structured nanomaterials are presented as an outstanding sensing platform because of their unique combination of high porosity in both the micro- and nanoscale, their biocompatibility, and flexible template applicability. Herein, we introduce a bacterial skeleton method allowing for cost-effective fabrication with nanoscale precision. As a proof-of-concept, we fabricated a hollow SnO2 hemipill network (HSHN) and a hollow Pt-functionalized SnO2 hemipill network (HPN). A superior detecting capability of HPN toward acetone, a diabetes biomarker, was demonstrated at low concentration (200 ppb) under high humidity (RH 80%). The detection limit reaches 3.6 ppb, a level satisfying the minimum requirement for diabetes breath diagnosis. High selectivity of the HPN sensor against C6H6, C7H8, CO, and NO vapors is demonstrated using principal component analysis (PCA), suggesting new applications of HPN for human-activity monitoring and a personal healthcare tool for diagnosing diabetes. The skeleton method can be further employed to mimic nanostructures of biomaterials with unique functionality for broad applications.

['Moon|Hi Gyu|HG|', 'Jung|Youngmo|Y|', 'Jun|Dukwoo|D|', 'Park|Ji Hyun|JH|', 'Chang|Young Wook|YW|', 'Park|Hyung-Ho|HH|0000-0001-5540-5433', 'Kang|Chong-Yun|CY|', 'Kim|Chulki|C|', 'Kaner|Richard B|RB|0000-0003-0345-4924']

2018

33246911

Long-term azithromycin use is not associated with QT prolongation in children with cystic fibrosis.

Chronic Azithromycin (AZM) is a common treatment for lung infection. Among adults at risk of cardiac events, AZM use has been associated with cardiovascular harm. We assessed cardiovascular safety of AZM among children with CF, as a secondary analysis of a placebo-controlled, clinical trial, in which study drug was taken thrice-weekly for a planned 18 months. Safety assessments using electrocardiogram (ECG) occurred at study enrollment, and then after 3 weeks and 18 months of participation. Among 221 study participants with a median of 18 months follow-up, increased corrected QT interval (QTc) of ≥30 msec was rare, at 3.4 occurrences per 100 person-years; and incidence of QTc prolongation was no higher in the AZM arm than the placebo arm (1.8 versus 5.4 per 100 person-years). No persons experienced QTc intervals above 500 msec. Long-term chronic AZM use was not associated with increased QT prolongation.

['Magaret|Amalia S|AS|', 'Salerno|Jack|J|', 'Deen|Jason F|JF|', 'Kloster|Margaret|M|', 'Mayer-Hamblett|Nicole|N|', 'Ramsey|Bonnie W|BW|', 'Nichols|Dave P|DP|']

2021

28069062

Quality of life in community-dwelling Dutch elderly measured by EQ-5D-3L.

BACKGROUND We aimed to evaluate health status and associated factors in community-dwelling elderly in the Netherlands. METHODS Participants from a placebo-controlled double-blind randomized controlled trial conducted in the Netherlands were invited at the time of enrolment to participate in this study. Data were collected on comorbidities, socio-demographic background and health status, using EQ-5D-3L instrument. EQ-5D-3L summary index values (EQ-5D-indices) was derived using Dutch tariff. Regression analysis was conducted to identify factors associated with EQ-5D-indices and visual analogue scale (EQ-VAS). RESULTS 48,634 elderly (≥65 years) were included. The most frequently reported complaint was pain/discomfort (29.4%), but for the elder elderly (i.e. ≥85 years) it was mobility (52.9%). The proportion of persons reporting (multiple) problems increased with age from 31.5% for 65-69 years old subjects to 65.9% for elder elderly. The mean EQ-5D-indices and EQ-VAS decreased with age from 0.94 and 84, respectively in those 65 to 69 years old to 0.86 and 76, respectively, in ≥85 years old subjects. Increasing age, female gender, low education, geographic factors and comorbidities were associated with impaired health status. CONCLUSIONS Within community-dwelling elderly large differences in health status exist. Impairment increases rapidly with age, but health status is also associated with socio-demographic variables and comorbidities. TRIAL REGISTRATION ClinicalTrials.gov, NCT00812084 .

['Mangen|Marie-Josée J|MJ|', 'Bolkenbaas|Marieke|M|', 'Huijts|Susanne M|SM|', 'van Werkhoven|Cornelis H|CH|', 'Bonten|Marc J M|MJ|', 'de Wit|G Ardine|GA|']

2017

33131644

The Nephrology Clinician Educator: Pathway and Future.

In the medical profession, teaching has always been a routine expectation for practicing physicians. While this remains true today, in recent years, we have seen the emergence of a well-defined career pathway for those practicing physicians who want to focus on education: the clinician educator. This is a physician who is highly active in the practice of teaching, science of learning, service as a role model for young physicians, and leading educational programs. In nephrology, one can have a fruitful and fulfilling career as a lifelong clinician educator. As career interest in our specialty wanes, the clinician educator is the professional well suited to reverse this trend. In this article, we will further define the clinician educator and map out a pathway of skills needed to thrive in this rewarding career. We also provide recommendations to both educators and leaders to ensure the clinician educator pathway continues to grow.

['Roberts|John K|JK|', 'Burgner|Anna M|AM|', 'Yau|Timothy|T|']

2020

31041727

Mid-term outcomes of simultaneous mitral valve repair in patients with miral regurgitation and concomitant annulo-aortic ectasia.

OBJECTIVE This study investigated the mid-term outcomes of simultaneous mitral valve repair in patients with mitral regurgitation (MR) and concomitant annulo-aortic ectasia. METHODS The study included 26 patients with MR and annulo-aortic ectasia [mean age 46.0 ± 19.9 (10-86) years] who underwent simultaneous mitral valve repair between January 2007 and March 2018. Of these 26 patients, 11 (42.3%) were diagnosed with Marfan syndrome and 10 (38.5%) with Barlow's disease. All patients underwent complete ring annuloplasty; a semi-rigid ring was used in 14 (53.8%) and a semi-flexible ring (anterior-flexible) in 12 patients (46.2%). All patients underwent valve-sparing root replacement using the reimplantation technique. RESULTS The overall 3-year survival rate was 95.7 ± 4.3%. The 3-year freedom from > moderate MR rate was 94.7 ± 5.1%, and the 3-year freedom from > moderate aortic regurgitation (AR) rate was 86.7 ± 7.3%. The 3-year freedom from reoperation rate was 100%. The 3-year freedom from > moderate MR rate was 100% in the semi-rigid ring group and 85.7 ± 13.2% in the semi-flexible ring group (log-rank test, p = 0.5371). The 3-year freedom from > moderate AR rate was 100% in the semi-rigid ring group and 72.9 ± 16.5% in the semi-flexible ring group (log-rank test, p = 0.0815). CONCLUSIONS Simultaneous mitral valve repair in patients with MR and concomitant annulo-aortic ectasia showed favorable mid-term outcomes.

['Koda|Yojiro|Y|', 'Kawamoto|Tatsuya|T|', 'Yokawa|Koki|K|', 'Henmi|Soichiro|S|', 'Nakai|Hidekazu|H|', 'Yamanaka|Katsuhiro|K|', 'Inoue|Takeshi|T|', 'Tanaka|Hiroshi|H|', 'Okita|Yutaka|Y|']

2019

32381822

Evaluation of Transverse Strength of Heat Cure Denture Bases Repaired with Different Joint Surface Contours: An In Vitro Study.

AIM The present in vitro study aimed to evaluate the transverse strength of heat cure acrylic denture base resins repaired with three different joint surface designs, repair material being the glass-fiber-reinforced self-cure repair material along with surface pretreatment. MATERIALS AND METHODS A total of 40 heat cure resin specimens were fabricated and finished to a size of 64 mm × 10 mm × 2.5 mm. These specimens were divided into four groups of 10 each, with butt, round, and bevel repair surface joints, respectively, along with the control group. After the specimens were cut in the middle for the repair procedure to proceed, the aforementioned joint surface contours were prepared for groups I, II, and III, respectively, treated with dimethyl chloride for 30 seconds, and the repair was done with E-glass-fiber-reinforced self-cure repair material. RESULTS The mean transverse strength of the control group was 100.37 N, which was highest (significant p value of <0.05), followed by group III (bevel joint) 54.23 N, group I (butt joint) 53.93 N, and group II (round joint) 44.55 N. CONCLUSION Distribution of stresses evenly in the repair region will be achieved by the proper selection of the joint surface contour, and this study showed that the 45° bevel joint was an appropriate selection along with promising repair material and surface pretreatment. CLINICAL SIGNIFICANCE Clinically, in the event of repairing a broken denture, a rapid and economically convenient material with superior physical and mechanical properties is important to maintain the adequate transverse strength of the repaired denture. Glass-fiber-reinforced autopolymerizing resin, with a 45° bevel joint design of the broken surfaces and surface pretreatment proved to reduce stress concentration, improving the transverse strength of the repaired heat cure denture base resins.

['Mamatha|Nallamilli|N|', 'Madineni|Praveen K|PK|', 'Sisir|Rapolu|R|', 'Sravani|Siripurapu|S|', 'Nallamilli|Saimadhavi|S|', 'Jyothy|Jorige R|JR|']

2020

32945462

lncRNA PCNAP1 predicts poor prognosis in breast cancer and promotes cancer metastasis via miR‑340‑5p‑dependent upregulation of SOX4.

The high metastatic rate of breast cancer is the significant cause of its poor prognosis. The long noncoding RNA (lncRNA) proliferating cell nuclear antigen pseudogene 1 (PCNAP1) plays important roles in the initiation and progression of cancers; however, its regulatory function and molecular mechanism in breast cancer metastasis remains unknown. Therefore, we investigated the roles of lncRNA PCNAP1 in breast cancer metastasis by modulating the microRNA (miR)‑340‑5p/SOX4 axis using quantitative real‑time PCR, in vivo mouse models, nucleo‑cytoplasmic separation, western blot analysis, scratch assays, Transwell assays, luciferase reporter assays and MS2‑RIP, in vitro and in vivo. lncRNA PCNAP1 was found to be upregulated in human breast cancer tissues, and high lncRNA PCNAP1 levels predicted poor overall survival. Function assays showed that knockdown of lncRNA PCNAP1 suppressed the migration and invasion of breast cancer cells in vitro and in vivo. Mechanistically, lncRNA PCNAP1 functioned as a competing endogenous (ce)RNA for miR‑340‑5p to facilitate the expression of its target gene SRY‑box transcription factor 4 (SOX4), promoting migration and invasion of breast cancer cells. Overall, we found that lncRNA PCNAP1 predicted a poor prognosis in breast cancer and promoted cancer metastasis via miR‑340‑5p‑dependent upregulation of SOX4 expression. These results suggest that lncRNA PCNAP1 has potential as an alternative therapeutic target to suppress breast cancer metastasis.

['Yu|Yang|Y|', 'He|Yaning|Y|', 'Shao|Yingbo|Y|', 'Chen|Qi|Q|', 'Liu|Hui|H|']

2020

27226011

Influence of Grape Maturity on Complex Carbohydrate Composition of Red Sparkling Wines.

This paper studied how grape maturity affected complex carbohydrate composition during red sparkling wine making and wine aging. Grape ripening stage (premature and mature grapes) showed a significant impact on the content, composition, and evolution of polysaccharides and oligosaccharides of sparkling wines. Polysaccharides rich in arabinose and galactose, mannoproteins, rhamnogalacturonans II, and oligosaccharides in base wines increased with maturity. For both maturity stages, polysaccharides rich in arabinose and galactose, and the glucuronic acid glycosyl residue of the oligosaccharides were the major carbohydrates detected in all vinification stages. The total glycosyl content of oligosaccharides decreased during the whole period of aging on yeast lees. The reduction of polysaccharides rich in arabinose and galactose and rhamnogalacturonans type II during the aging was more pronounced in mature samples. To our knowledge, this is the first report of the polysaccharide and oligosaccharide composition of red sparkling wines.

['Martínez-Lapuente|Leticia|L|', 'Apolinar-Valiente|Rafael|R|', 'Guadalupe|Zenaida|Z|', 'Ayestarán|Belén|B|', 'Pérez-Magariño|Silvia|S|', 'Williams|Pascale|P|', 'Doco|Thierry|T|']

2016

32029124

Electrophysiology-based stratification of pancreatic tumorigenicity by label-free single-cell impedance cytometry.

Pancreatic ductal adenocarcinoma (PDAC) is an aggressive cancer lacking specific biomarkers that can be correlated to disease onset, promotion and progression. To assess whether tumor cell electrophysiology may serve as a marker for PDAC tumorigenicity, we use multi-frequency impedance cytometry at high throughput (∼350 cells/s) to measure the electrical phenotype of single PDAC tumor cells from xenografts, which are derived from primary pancreatic tumors versus those from liver metastases of different patients. A novel phase contrast metric based on variations in the high and low frequency impedance phase responses that is related to electrophysiology of the cell interior is found to be systematically altered as a function of tumorigenicity. PDAC cells of higher tumorigenicity exhibited lowered interior conductivity and enhanced permittivity, which is validated by the dielectrophoresis on the respective cell types. Using genetic analysis, we suggest the role of dysregulated Na+ transport and removal of Ca2+ ions from the cytoplasm on key oncogenic KRAS-driven processes that may be responsible for lowering of the interior cell conductivity. We envision that impedance cytometry can serve as a tool to quantify phenotypic heterogeneity for rapidly stratifying tumorigenicity. It can also aid in protocols for dielectrophoretic isolation of cells with a particular phenotype for prognostic studies on patient survival and to tailor therapy selection to specific patients.

['McGrath|J S|JS|', 'Honrado|C|C|', 'Moore|J H|JH|', 'Adair|S J|SJ|', 'Varhue|W B|WB|', 'Salahi|A|A|', 'Farmehini|V|V|', 'Goudreau|B J|BJ|', 'Nagdas|S|S|', 'Blais|E M|EM|', 'Bauer|T W|TW|', 'Swami|N S|NS|']

2020

32571379

Radiobiological model-based approach to determine the potential of dose-escalated robust intensity-modulated proton radiotherapy in reducing gastrointestinal toxicity in the treatment of locally advanced unresectable pancreatic cancer of the head.

BACKGROUND The purpose of this study was to determine the potential of escalated dose radiation (EDR) robust intensity-modulated proton radiotherapy (ro-IMPT) in reducing GI toxicity risk in locally advanced unresectable pancreatic cancer (LAUPC) of the head in term of normal tissue complication probability (NTCP) predictive model. METHODS For 9 patients, intensity-modulated radiotherapy (IMRT) was compared with ro-IMPT. For all plans, the prescription dose was 59.4GyE (Gray equivalent) in 33 fractions with an equivalent organ at risk (OAR) constraints. Physical dose distribution was evaluated. GI toxicity risk for different endpoints was estimated using published NTCP Lyman Kutcher Burman (LKB) models for stomach, duodenum, small bowel, and combine stomach and duodenum (Stoduo). A Wilcoxon signed-rank test was used for dosimetry parameters and NTCP values comparison. RESULT The dosimetric results have shown that, with similar target coverage, ro-IMPT achieves a significant dose-volume reduction in the stomach, small bowel, and stoduo in low to high dose range in comparison to IMRT. NTCP evaluation for the endpoint gastric bleeding of stomach (10.55% vs. 13.97%, P = 0.007), duodenum (1.87% vs. 5.02%, P = 0.004), and stoduo (5.67% vs. 7.81%, P = 0.008) suggest reduced toxicity by ro-IMPT compared to IMRT. ∆NTCP IMRT - ro-IMPT (using parameter from Pan et al. for gastric bleed) of ≥5 to < 10% was seen in 3 patients (33%) for stomach and 2 patients (22%) for stoduo. An overall GI toxicity relative risk (NTCPro-IMPT/NTCPIMRT) reduction was noted (0.16-0.81) for all GI-OARs except for duodenum (> 1) with endpoint grade ≥ 3 GI toxicity (using parameters from Holyoake et al.). CONCLUSION With similar target coverage and better conformity, ro-IMPT has the potential to substantially reduce the risk of GI toxicity compared to IMRT in EDR of LAUPC of the head. This result needs to be further evaluated in future clinical studies.

['Raturi|Vijay P|VP|', 'Hojo|Hidehiro|H|', 'Hotta|Kenji|K|', 'Baba|Hiromi|H|', 'Takahashi|Ryo|R|', 'Rachi|Toshiya|T|', 'Nakamura|Naoki|N|', 'Zenda|Sadamoto|S|', 'Motegi|Atsushi|A|', 'Tachibana|Hidenobu|H|', 'Ariji|Takaki|T|', 'Motegi|Kana|K|', 'Nakamura|Masaki|M|', 'Okumura|Masayuki|M|', 'Hirano|Yasuhiro|Y|', 'Akimoto|Tetsuo|T|']

2020

31803961

From clinical specimens to human cancer preclinical models-a journey the NCI-cell line database-25 years later.

The intramural the National Cancer Institute (NCI) and more recently the University of Texas Southwestern Medical Center with many different collaborators comprised a complex, multi-disciplinary team that collaborated to generated large, comprehensively annotated, cell-line related research resources which includes associated clinical, and molecular characterization data. This material has been shared in an anonymized fashion to accelerate progress in overcoming lung cancer, the leading cause of cancer death across the world. However, this cell line collection also includes a range of other cancers derived from patient-donated specimens that have been remarkably valuable for other types of cancer and disease research. A comprehensive analysis conducted by the NCI Center for Research Strategy of the 278 cell lines reported in the original Journal of Cellular Biochemistry Supplement, documents that these cell lines and related products have since been used in more than 14 000 grants, and 33 207 published scientific reports. This has resulted in over 1.2 million citations using at least one cell line. Many publications involve the use of more than one cell line, to understand the value of the resource collectively rather than individually; this method has resulted in 2.9 million citations. In addition, these cell lines have been linked to 422 clinical trials and cited by 4700 patents through publications. For lung cancer alone, the cell lines have been used in the research cited in the development of over 70 National Comprehensive Cancer Network clinical guidelines. Finally, it must be underscored again, that patient altruism enabled the availability of this invaluable research resource.

['Mulshine|James L|JL|http://orcid.org/0000-0002-4648-9177', 'Ujhazy|Peter|P|', 'Antman|Melissa|M|', 'Burgess|Christine M|CM|', 'Kuzmin|Igor|I|', 'Bunn|Paul A|PA|', 'Johnson|Bruce E|BE|', 'Roth|Jack A|JA|', 'Pass|Harvey I|HI|', 'Ross|Sheila M|SM|', 'Aldige|Carolyn R|CR|', 'Wistuba|Ignacio I|II|', 'Minna|John D|JD|']

2019

28422069

Protein Stability and Unfolding Following Glycine Radical Formation.

Glycine (Gly) residues are particularly susceptible to hydrogen abstraction; which results in the formation of the capto-dative stabilized Cα-centered Gly radical (GLR) on the protein backbone. We examined the effect of GLR formation on the structure of the Trp cage; tryptophan zipper; and the villin headpiece; three fast-folding and stable miniproteins; using all-atom (OPLS-AA) molecular dynamics simulations. Radicalization changes the conformation of the GLR residue and affects both neighboring residues but did not affect the stability of the Trp zipper. The stability of helices away from the radical center in villin were also affected by radicalization; and GLR in place of Gly15 caused the Trp cage to unfold within 1 µs. These results provide new evidence on the destabilizing effects of protein oxidation by reactive oxygen species.

['Owen|Michael C|MC|', 'Csizmadia|Imre G|IG|', 'Viskolcz|Béla|B|', 'Strodel|Birgit|B|']

2017

24997947

Seasonal changes in antibiotics, antidepressants/psychiatric drugs, antihistamines and lipid regulators in a wastewater treatment plant.

Seasonal changes in the concentration of 21 pharmaceuticals in a wastewater treatment plant (WWTP) in České Budějovice were investigated over 12months. The target compounds were 10 antibiotics, 4 antidepressants, 3 psychiatric drugs, 2 antihistamines and 2 lipid regulators. 272 Wastewater samples (136 influents and 136 effluents) were collected from March 2011 to February 2012 and analyzed using two-dimensional liquid chromatography coupled with tandem mass spectrometry. All studied pharmaceuticals were frequently detected in both the influent and the effluent wastewater samples, except for meclozine, which was only found in the influent. The mean concentration of pharmaceuticals varied from 0.006μgL(-1) to 1.48μgL(-1) in the influent and from 0.003μgL(-1) to 0.93μgL(-1) in the effluent. The concentration of most pharmaceuticals was higher during winter.

['Golovko|Oksana|O|', 'Kumar|Vimal|V|', 'Fedorova|Ganna|G|', 'Randak|Tomas|T|', 'Grabic|Roman|R|']

2014

28104981

Sodium butyrate attenuates high-fat diet-induced steatohepatitis in mice by improving gut microbiota and gastrointestinal barrier.

AIM To investigate whether gut microbiota metabolite sodium butyrate (NaB) is an effective substance for attenuating non-alcoholic fatty liver disease (NAFLD) and the internal mechanisms. METHODS Male C57BL/6J mice were divided into three groups, normal control were fed standard chow and model group were fed a high-fat diet (HFD) for 16 wk, the intervention group were fed HFD for 16 wk and treated with NaB for 8 wk. Gut microbiota from each group were detected at baseline and at 16 wk, liver histology were evaluated and gastrointestinal barrier indicator such as zonula occluden-1 (ZO-1) were detected by immunohistochemistry and realtime-PCR, further serum or liver endotoxin were determined by ELISA and inflammation- or metabolism-associated genes were quantified by real-time PCR. RESULTS NaB corrected the HFD-induced gut microbiota imbalance in mice, while it considerably elevated the abundances of the beneficial bacteria Christensenellaceae, Blautia and Lactobacillus. These bacteria can produce butyric acid in what seems like a virtuous circle. And butyrate restored HFD induced intestinal mucosa damage, increased the expression of ZO-1 in small intestine, further decreased the levels of gut endotoxin in serum and liver compared with HF group. Endotoxin-associated genes such as TLR4 and Myd88, pro-inflammation genes such as MCP-1, TNF-α, IL-1, IL-2, IL-6 and IFN-γ in liver or epididymal fat were obviously downregulated after NaB intervention. Liver inflammation and fat accumulation were ameliorated, the levels of TG and cholesterol in liver were decreased after NaB intervention, NAS score was significantly decreased, metabolic indices such as FBG and HOMA-IR and liver function indicators ALT and AST were improved compared with HF group. CONCLUSION NaB may restore the dysbiosis of gut microbiota to attenuate steatohepatitis, which is suggested to be a potential gut microbiota modulator and therapeutic substance for NAFLD.

['Zhou|Da|D|', 'Pan|Qin|Q|', 'Xin|Feng-Zhi|FZ|', 'Zhang|Rui-Nan|RN|', 'He|Chong-Xin|CX|', 'Chen|Guang-Yu|GY|', 'Liu|Chang|C|', 'Chen|Yuan-Wen|YW|', 'Fan|Jian-Gao|JG|']

2017

31901730

Hair cortisol as a biomarker of stress and resilience in South African mixed ancestry females.

BACKGROUND Hair cortisol concentrations (HCC) are increasingly used as a biomarker of stress, however limited research exists regarding the relationship between HCC and protective factors, such as resilience. Additionally, studies measuring HCC need to account for possible confounders, and these factors have not been examined in sufficiently diverse settings. OBJECTIVES Our objectives were to identify determinants of HCC in a sample of mixed ancestry adults and investigate the association of HCC with measures of self-perceived stress and resilience. METHODS Our sample comprised 164 females (mean age 46.5 years, SD = 15.0), self-identifying as mixed ancestry, who were control participants in a case-control study (SHARED ROOTS), conducted in Cape Town, South Africa from May 2014 until June 2017. We examined which socio-demographic, hair related, clinical and behavioural factors were associated with HCC in both unadjusted and adjusted linear regression models. Furthermore, the relationship of HCC with self-perceived stress and resilience scores were also examined. RESULTS HCC (Mdn 4.4 pg/ml; IQR 2.8; 11.4) were significantly positively associated with hair product use and breastfeeding, and significantly negatively associated with age, level of education, duration of sun exposure, duration of storage, and demonstrated a trend towards significance with frequency of hair washing, in adjusted models. HCC were inversely associated with CD-RISC scores (adj β = -0.179, p =  0.012) but were not significantly associated with PSS scores (adj β = -0.001, p =  0.989). CONCLUSIONS We identified specific determinants of HCC in our sample, including the first indication that sun exposure has an effect on HCC under naturalistic conditions. These potential confounders need to be controlled for in the design and analysis of future studies. HCC may be a biomarker of resilience to stress, rather than perceived stress. Further research measuring HCC in more diverse settings and populations and including constructs related to resilience are needed.

['van den Heuvel|Leigh Luella|LL|', 'Acker|Debbie|D|', 'du Plessis|Stéfan|S|', 'Stalder|Tobias|T|', 'Suliman|Sharain|S|', 'Thorne|Marguerite Yvonne|MY|', 'Kirschbaum|Clemens|C|', 'Seedat|Soraya|S|']

2020

30405936

Estrogen reinforces barrier formation and protects against tumor necrosis factor alpha-induced barrier dysfunction in oral epithelial cells.

Purpose Epithelial barrier dysfunction is involved in the pathophysiology of periodontitis and oral lichen planus. Estrogens have been shown to enhance the physical barrier function of intestinal and esophageal epithelia, and we aimed to investigate the effect of estradiol (E2) on the regulation of physical barrier and tight junction (TJ) proteins in human oral epithelial cell monolayers. Methods HOK-16B cell monolayers cultured on transwells were treated with E2, an estrogen receptor (ER) antagonist (ICI 182,780), tumor necrosis factor alpha (TNFα), or dexamethasone (Dexa), and the transepithelial electrical resistance (TER) was then measured. Cell proliferation was measured by the cell counting kit (CCK)-8 assay. The levels of TJ proteins and nuclear translocation of nuclear factor (NF)-κB were examined by confocal microscopy. Results E2 treatment increased the TER and the levels of junctional adhesion molecule (JAM)-A and zonula occludens (ZO)-1 in a dose-dependent manner, without affecting cell proliferation during barrier formation. Treatment of the tight-junctioned cell monolayers with TNFα induced decreases in the TER and the levels of ZO-1 and nuclear translocation of NF-κB. These TNFα-induced changes were inhibited by E2, and this effect was completely reversed by co-treatment with ICI 182,780. Furthermore, E2 and Dexa presented an additive effect on the epithelial barrier function. Conclusions E2 reinforces the physical barrier of oral epithelial cells through the nuclear ER-dependent upregulation of TJ proteins. The protective effect of E2 on the TNFα-induced impairment of the epithelial barrier and its additive effect with Dexa suggest its potential use to treat oral inflammatory diseases involving epithelial barrier dysfunction.

['Choi|Yun Sik|YS|https://orcid.org/0000-0002-1321-8119', 'Baek|Keumjin|K|https://orcid.org/0000-0002-4849-0083', 'Choi|Youngnim|Y|https://orcid.org/0000-0002-6496-5560']

2018

32914616

Highly Conductive and Flexible Dopamine-Graphene Hybrid Electronic Textile Yarn for Sensitive and Selective NO2 Detection.

Graphene-based electronic textile (e-textile) gas sensors have been developed for detecting hazardous NO2 gas. For the e-textile gas sensor, electrical conductivity is a critical factor because it directly affects its sensitivity. To obtain a highly conductive e-textile, biomolecules have been used for gluing the graphene to the textile surface, though there remain areas to improve, such as poor conductivity and flexibility. Herein, we have developed a dopamine-graphene hybrid electronic textile yarn (DGY) where the dopamine is used as a bio-inspired adhesive to attach graphene to the surface of yarns. The DGY shows improved electrical conductivity (∼40 times) compared to conventional graphene-based e-textile yarns with no glue. Moreover, it exhibited improved sensing performance in terms of short response time (∼2 min), high sensitivity (0.02 μA/ppm), and selectivity toward NO2. The mechanical flexibility and durability of the DGY were examined through a 1000-cycle bending test. For a practical application, the DGY was attempted to detect the NO emitted from vehicles, including gasoline, diesel, and fuel cell electric vehicles. Our results demonstrated that the DGYs-as a graphene-based e-textile gas sensor for detecting NO2-are simple to fabricate, cheap, disposable, and mechanically stable.

['Lee|Sang Won|SW|http://orcid.org/0000-0001-5850-6555', 'Jung|Hyo Gi|HG|', 'Kim|Insu|I|http://orcid.org/0000-0001-5359-0890', 'Lee|Dongtak|D|http://orcid.org/0000-0001-8716-1736', 'Kim|Woong|W|', 'Kim|Sang Hun|SH|', 'Lee|Jong-Heun|JH|http://orcid.org/0000-0002-3075-3623', 'Park|Jinsung|J|', 'Lee|Jeong Hoon|JH|', 'Lee|Gyudo|G|', 'Yoon|Dae Sung|DS|']

2020

25841998

Risk factors for foot and mouth disease outbreaks in grazing beef cattle herds.

Foot and mouth disease (FMD) is considered one of the most important diseases of cattle. Recurrence of FMD outbreaks in Israel is common, even though routine vaccination of livestock is mandatory and control measures are applied during the outbreaks. Grazing beef herds are occasionally involved in these outbreaks and play an important role in disseminating the disease, due to the large efflux of animals from these herds to feedlots. Nevertheless, the risk factors for the occurrence of FMD among these herds have never been investigated. In 2011, Israel faced a large scale outbreak of serotype O FMD virus, which strongly affected beef cattle. We conducted a case-control study of 44 beef cattle herds grazing in the Golan Heights in order to determine the risk factors for FMDV infection. Data were analyzed using a generalized estimation equation (GEE) with a logit link function. Multivariable analysis was conducted for factors with p-value lower than 0.1 in the univariable analysis. The presence of calves under 6 months of age was found as a significant risk factor for FMDV infection in the univariable analysis (odds ratio (OR)=5.95, confidence intervals of 95% (CI95%)=1.59-22.29, p=0.008). This was also the only variable that remained statistically significant in the multivariable analysis. Herds in which more than 6 months between vaccination of adults and exposure had elapsed were in higher risk, albeit not statistically significant, for the occurrence of FMDV infection (OR=3.29, CI95%=0.83-12.99, p=0.089). The higher probability of infection in herds, which included young calves may be a result of their higher susceptibility due to administration of only one or no vaccine prior to the outbreak. The results of the study thus support increasing the frequency of vaccination of both cows and calves in grazing beef herds. Intensifying surveillance where young calves are abundant may also prove efficient for early detection of infected herds and for mitigating outbreaks of FMDV.

['Elnekave|E|E|', 'Zamir|L|L|', 'Hamd|F|F|', 'Even Tov|B|B|', 'Klement|E|E|']

2015

32306869

Human olfactory communication: current challenges and future prospects.

Although anthropologists frequently report the centrality of odours in the daily lives and cultural beliefs of many small-scale communities, Western scholars have historically considered the sense of smell as minimally involved in human communication. Here, we suggest that the origin and persistence of this latter view might be a consequence of the fact that most research is conducted on participants from Western societies who, collectively, were rather old (adults), deodorized and desensitized (ODD) to various aspects of olfactory perception. The view is rapidly changing, however, and this themed issue provides a timely overview of the current state-of-the-art on human chemocommunication. Based on evolutionary models of communication, the papers cover both general mechanisms of odour production by 'senders' and odour perception by 'receivers'. Focus on specific functional contexts includes reciprocal impact of odours between infants and mothers, the role of odour in mate choice and how odours communicate emotion and disease. Finally, a position paper outlines pitfalls and opportunities for the future, against the context of the replication crisis in psychology. We believe a more nuanced view of human chemical communication is within our grasp if we can continue to develop inter-disciplinary insights and expand research activities beyond ODD people. This article is part of the Theo Murphy meeting issue 'Olfactory communication in humans'.

['Roberts|S Craig|SC|', 'Havlíček|Jan|J|', 'Schaal|Benoist|B|']

2020

32173179

Patient-Reported Outcome Measures (PROMs): Influence of Motor Tasks and Psychosocial Factors on FAAM Scores in Foot and Ankle Trauma Patients.

Patient-reported outcome measures (PROMS) are being increasingly used as a quality of care metric. However, the validity and consistency of PROMS remain undefined. The study sought to determine whether Foot and Ankle Ability Measure (FAAM) scores improve after patients complete motor tasks evaluated on the survey and to examine the relationship between depression and self-efficacy and FAAM scores or change in scores. We conducted a prospective comparison study of adults with isolated foot, ankle, or distal tibia fractures treated operatively at level I trauma center. Twenty-seven patients completed the FAAM survey at the first clinic visit after being made weightbearing as tolerated (mean 3 months). Patients then completed 6 motor tasks queried on FAAM (standing, walking without shoes, squatting, stairs, up to toes), followed by a repeat FAAM and General Self-Efficacy scale (GSE) and Patient Health Questionnaire-2 (PHQ-2) instruments. FAAM scores before and after intervention; GSE and PHQ-2 scores compared with baseline FAAM and change in FAAM scores. Performing motor tasks significantly improved postintervention scores for squatting (P = .044) and coming up to toes (P = .012), the 2 most strenuous tasks. No difference was found for the remaining tasks. Higher depression ratings correlated with worse FAAM scores overall (P < .05). Higher self-efficacy ratings correlated with increase in FAAM Sports subscale postintervention (P = .020). FAAM scores are influenced by performing motor tasks. Self-reported depression influences baseline FAAM scores and self-efficacy may influence change in FAAM scores. Context and patient factors (modifiable and nonmodifiable) affect PROM implementation, with implications for clinical care, reimbursement models, and use of quality measure.

['Schultz|Blake J|BJ|', 'Tanner|Natalie|N|', 'Shapiro|Lauren M|LM|', 'Segovia|Nicole A|NA|', 'Kamal|Robin N|RN|', 'Bishop|Julius A|JA|', 'Gardner|Michael J|MJ|']

2020

32270724

Association between basal proteinuria levels and pregnancy outcomes in familial Mediterranean fever.

We aimed to investigate whether proteinuria in the first trimester of pregnancy in Familial Mediterranean fever (FMF) patients has an impact on pregnancy outcome and perinatal and neonatal outcome of pregnancies. A total of 66 pregnant with FMF were compared with healthy controls at the same gestational weeks. Patients with FMF had a higher antenatal hospitalisation rate (34.8% vs. 6.1%, respectively, p < .01) and higher rate of 2 or more miscarriages. FMF patients with or without obstetric complications also had a similar amount of 24-h urine proteinuria in the first trimester. Patients on colchicine therapy during pregnancy had more frequent attacks in pregnancy (59.3% vs. 18.2%, respectively, p: .012). The rates of preeclampsia, preterm delivery, foetal anomalies, small for gestation age neonates and primary caesarean rate were similar between groups. In conclusion; FMF had no significant impact on pregnancy. Neither attacks in pregnancy nor basal proteinuria were associated with adverse outcomes.Impact statementWhat is already known on this subject? Familial Mediterranean fever (FMF) is an autosomal recessive disease characterised by inflammation of the serosal, synovial and cutaneous tissues with recurrent attacks. One of the most serious complications of FMF is amyloidosis that can cause end-stage renal disease. Outcomes of FMF on pregnancy have been analysed by only few studies. Amyloidosis based on the initial renal function may adversely affect pregnancies. It has been reported that FMF patients with renal amyloidosis may suffer pregnancy complications to a greater extent.What do the results of this study add? There have been few studies on the correlation between FMF, proteinuria and pregnancy outcomes. In our study we found that FMF had no significant impact on pregnancy. Neither attacks in pregnancy nor basal proteinuria were associated with adverse outcomes.What are the implications of these findings for clinical practice and/or further research? Our study suggested that FMF had no relationship between pregnancy outcomes. However, our study population is relatively small. It will contribute to comprehensive studies involving a larger population. Future studies should be performed to investigate the effects of basal proteinuria in pregnancy with FMF.

['Iskender|D|D|', 'Kara|O|O|', 'Ozturk Kaymak|A|A|', 'Daglar|H K|HK|', 'Kirbas|A|A|', 'Iskender|C T|CT|', 'Kaymak|O|O|', 'Celen|S|S|']

2020

31344673

Use of Smartphone-Based Head-Mounted Display Devices to View a Three-Dimensional Dissection Model in a Virtual Reality Environment: Pilot Questionnaire Study.

BACKGROUND Virtual reality (VR) technology has started to gain attention as a form of surgical support in medical settings. Likewise, the widespread use of smartphones has resulted in the development of various medical applications; for example, Google Cardboard, which can be used to build simple head-mounted displays (HMDs). However, because of the absence of observed and reported outcomes of the use of three-dimensional (3D) organ models in relevant environments, we have yet to determine the effects of or issues with the use of such VR technology. OBJECTIVE The aim of this paper was to study the issues that arise while observing a 3D model of an organ that is created based on an actual surgical case through the use of a smartphone-based simple HMD. Upon completion, we evaluated and gathered feedback on the performance and usability of the simple observation environment we had created. METHODS We downloaded our data to a smartphone (Galaxy S6; Samsung, Seoul, Korea) and created a simple HMD system using Google Cardboard (Google). A total of 17 medical students performed 2 experiments: an observation conducted by a single observer and another one carried out by multiple observers using a simple HMD. Afterward, they assessed the results by responding to a questionnaire survey. RESULTS We received a largely favorable response in the evaluation of the dissection model, but also a low score because of visually induced motion sickness and eye fatigue. In an introspective report on simultaneous observations made by multiple observers, positive opinions indicated clear image quality and shared understanding, but displeasure caused by visually induced motion sickness, eye fatigue, and hardware problems was also expressed. CONCLUSIONS We established a simple system that enables multiple persons to observe a 3D model. Although the observation conducted by multiple observers was successful, problems likely arose because of poor smartphone performance. Therefore, smartphone performance improvement may be a key factor in establishing a low-cost and user-friendly 3D observation environment.

['Masuoka|Yoshihito|Y|http://orcid.org/0000-0002-7979-5787', 'Morikawa|Hiroyuki|H|http://orcid.org/0000-0002-8280-9553', 'Kawai|Takashi|T|http://orcid.org/0000-0002-9407-1197', 'Nakagohri|Toshio|T|http://orcid.org/0000-0001-6843-8891']

2019

29288086

Autoimmune hepatitis association with single nucleotide polymorphism of interleukin-2, but not interferon-gamma.

BACKGROUND Autoimmune hepatitis (AIH) is a chronic inflammation in hepatocellular tissues associated with circulating autoantibodies. Imbalance in T-cells population and dysregulation in several cytokine profiles has been implicated in pathogenesis of AIH. This study was performed to assess potential association of AIH with interleukin-2 (IL-2) and interferon-gamma (IFN-γ) genes single nucleotide polymorphisms (SNPs). METHODS Fifty-six patients with AIH and 139 healthy individuals were enrolled in this study. IL-2 and IFN-γ typing was performed, using polymerase chain reaction with sequence-specific primers (PCR-SSP) assay. The frequencies of alleles, genotypes and haplotypes in AIH patients were compared to healthy controls. RESULTS IL-2 T allele at position +166 (rs2069763) showed significant higher frequency in AIH group (36%), compared to the controls (21%) (OR=2.06; 95% CI, 1.24-3.43, P-value<0.01). The frequency of IL-2 TT genotype at +166 position was also associated with AIH (OR=18.68, 95% CI 3.74-126.04, P-value<0.01). G/T alleles of IL-2 at -330 (rs2069762) and A/T alleles on UTR +5644 position at IFN-γ and their subsequent haplotypes, did not show significant association with AIH. CONCLUSIONS This study identified IL-2T allele at +166 position and TT genotype as susceptibility gene in AIH which would provide better understandings into the mechanisms of AIH and potential immune modulation therapies.

['Yousefi|Azizollah|A|', 'Mahmoudi|Elham|E|', 'Baradaran Noveiry|Behnoud|B|', 'Zare Bidoki|Alireza|A|', 'Sadr|Maryam|M|', 'Motamed|Farzaneh|F|', 'Najafi|Mehri|M|', 'Farahmand|Fatemeh|F|', 'Khodadad|Ahmad|A|', 'Fallahi|Gholam Hossein|GH|', 'Rezaei|Nima|N|']

2018

33770497

HIC1 Represses Atoh1 Transcription and Hair Cell Differentiation in the Cochlea.

Across species, expression of the basic helix-loop-helix transcription factor ATOH1 promotes differentiation of cochlear supporting cells to sensory hair cells required for hearing. In mammals, this process is limited to development, whereas nonmammalian vertebrates can also regenerate hair cells after injury. The mechanistic basis for this difference is not fully understood. Hypermethylated in cancer 1 (HIC1) is a transcriptional repressor known to inhibit Atoh1 in the cerebellum. We therefore investigated its potential role in cochlear hair cell differentiation. We find that Hic1 is expressed throughout the postnatal murine cochlear sensory epithelium. In cochlear organoids, Hic1 knockdown induces Atoh1 expression and promotes hair cell differentiation, while Hic1 overexpression hinders differentiation. Wild-type HIC1, but not the DNA-binding mutant C521S, suppresses activity of the Atoh1 autoregulatory enhancer and blocks its responsiveness to β-catenin activation. Our findings reveal the importance of HIC1 repression of Atoh1 in the cochlea, which may be targeted to promote hair cell regeneration.

['Abdul-Aziz|Dunia|D|', 'Hathiramani|Nicolai|N|', 'Phung|Lauren|L|', 'Sykopetrites|Vittoria|V|', 'Edge|Albert S B|ASB|']

2021

34348048

Occurrence of Carriage of Multidrug Resistant Enterobacteriaceae among Pregnant Women in the Primary Health Center and Hospital Setting in Surabaya, Indonesia.

The incidence of healthy individuals carrying multidrug resistant Enterobacteriaceae, including extended-spectrum β-lactamase producing Enterobacteriaceae (ESBL-E), especially extended-spectrum β-lactamase producing Escherichia coli (ESBL-EC) and extended-spectrum β-lactamase producing Klebsiella pneumoniae (ESBL-KP), is increasing worldwide. Although ESBL-E causes early or late onset of neonatal sepsis, the prevalence of ESBL-E carriage among pregnant women in Indonesia is not clear. In the present study, we compared the occurrence of carriage of ESBL-E among pregnant women in a primary health center (PHC) versus two hospitals. We collected rectal swab samples from 200 pregnant women who visited a PHC or were admitted to two hospitals in Surabaya, Indonesia from July to October 2018. The ESBL-E strains were isolated from the samples and phenotypically and genotypically analyzed. ESBL-E strains were isolated from 25 (24.8%) pregnant women who visited the PHC and 49 (49.5%) pregnant women who were admitted to the hospitals. The rate of ESBL-E carriage of pregnant women in the hospitals was significantly higher than that in the PHC. Among the 74 isolated ESBL-E strains, ESBL-EC was most frequently isolated (62 strains), followed by ESBL-KP (12 strains). In addition, bla was the most frequent ESBL gene type of the isolated ESBL-E strains. Our results revealed the high occurrence of ESBL-E carriage in pregnant women, especially those who were admitted to the hospitals.

['Oktaviani Sulikah|Siti Rochmanah|SR|', 'Hasanah|Miratul|M|', 'Setyarini|Wahyu|W|', 'Parathon|Hari|H|', 'Kitagawa|Koichi|K|', 'Nakanishi|Noriko|N|', 'Nomoto|Ryohei|R|', 'Osawa|Kayo|K|', 'Kinoshita|Shohiro|S|', 'Hirai|Itaru|I|', 'Shirakawa|Toshiro|T|https://orcid.org/0000-0003-3925-8730', 'Kuntaman|Kuntaman|K|']

2021

30777360

Male-female relationships in olive baboons (Papio anubis): Parenting or mating effort?

Long-term male-female bonds and bi-parental investment in offspring are hallmarks of human society. A key question is how these traits evolved from the polygynandrously mating multimale multifemale society that likely characterized the Pan-Homo ancestor. In all three species of savanna baboons, lactating females form strong ties (sometimes called "friendships") with one or more adult males. For yellow baboons (Papio cynocephalus) and chacma baboons (Papio ursinus), several lines of evidence suggest that these relationships are a form of male parenting effort. In olive baboons (Papio anubis), females are thought to preferentially mate with their "friends", and male-female bonds may thus function as a form of mating effort. Here, we draw on behavioral and genetic data to evaluate the factors that shape male-female relationships in a well-studied population of olive baboons. We find support for the parenting effort hypothesis in that sires have stronger bonds with their infants' mothers than do other males. These bonds sometimes persist past weaning age and, in many cases, the sire of the previous infant is still a close partner of the female when she nurses her subsequent offspring. We find that males who have the strongest bonds with females that have resumed cycling, but are not currently sexually receptive, are more likely to sire the female's next offspring but the estimate is associated with large statistical uncertainty. We also find that in over one third of the cases, a female's successive infants were sired by the same male. Thus, in olive baboons, the development of stable breeding bonds and paternal investment seem to be grounded in the formation of close ties between males and anestrous females. However, other factors such as male dominance rank also influence paternity success and may preclude stability of these bonds to the extent found in human societies.

['Städele|Veronika|V|', 'Roberts|Eila R|ER|', 'Barrett|Brendan J|BJ|', 'Strum|Shirley C|SC|', 'Vigilant|Linda|L|', 'Silk|Joan B|JB|']

2019

27998664

Short-term complications after surgically assisted rapid palatal expansion: a retrospective cohort study.

Surgically assisted rapid palatal expansion (SARPE) has been considered a safe procedure with minimal patient morbidity. The aim of this study was to identify short-term complications encountered after tooth-borne expansion with a standardized approach to inform surgeons and orthodontists of the patient risk. In this retrospective cohort study, 55 patients (35 female, 20 male) undergoing SARPE between January 2013 and December 2014 were evaluated. Twenty-eight patients developed one or more complications. Postoperative haemorrhage was seen in six patients. Sixteen patients presented with injury to the infraorbital nerve, five had dental complications, and four had severe postoperative pain. A prolonged hospital stay was necessary for six patients and additional surgery was required in two cases. It is concluded that the short-term complications after tooth-borne SARPE are generally mild; however, the number of complications encountered indicates that SARPE is not free of risk and should be preceded by careful patient selection and planning.

['Verquin|M|M|', 'Daems|L|L|', 'Politis|C|C|']

2017

32634111

Impact of COVID-19 on gaming disorder: Monitoring and prevention.

The global coronavirus disease 2019 (COVID-19) outbreak has necessitated physical distancing, lockdown, contact tracing, and self-quarantine so as to prevent the spread of the disease. Amid the outbreak, gaming data usage has reportedly increased in the United States, and game download volume has reached a record high in Europe. Because gaming can be used to cope with the psychological stress from the outbreak, therefore mental health professionals should be aware of how increased gaming during the pandemic may contribute to risk of gaming disorder, especially if the pandemic persists. Mental health professionals should thus formulate safe social interaction alternatives for people, particularly adolescents, who have gaming disorder risk.

['Ko|Chih-Hung|CH|0000-0001-8034-0221', 'Yen|Ju-Yu|JY|']

2020

29881720

Screening, Diagnosis, and Management of Patients With Alcohol Use Disorders at Bwindi Community Hospital, Uganda.

Introduction: The harmful use of alcohol is a growing global public health concern, with Sub-Saharan Africa at particular risk. A large proportion of adults in Uganda consume alcohol and the country has a high prevalence of alcohol use disorders (AUD), almost double that for the African region as a whole. Bwindi Community Hospital, in rural western Uganda, recently introduced a program of screening, diagnosis and management of AUD and we assessed how this worked. Methods: This was a cross-sectional study in three departments (out-patients, adult in-patients and sexual & reproductive health) of Bwindi Community Hospital assessing numbers of patients screened, diagnosed and treated with AUD between January 2014 and June 2017. Data sources included the hospital electronic data base and departmental case files. Frequencies and proportions are reported and odds ratios used to compare specific factors associated with medical interventions. Results: Altogether, 82,819 patients attended or were admitted to hospital, of whom 8,627 (10.4%) were screened and 273 (3.2%) diagnosed with AUD. The adult in-patient department recorded the largest number with AUD (n = 206) as well as a consistent increase in numbers in the last 18 months of the study. Of those with AUD, there were 230 (84%) males, 130 (48%) aged 36-60 years, and 131 (48%) with medical non-alcohol related diagnostic categories. Medical/supportive interventions included guidance and counselling to 168 (62%), community social support to 90 (33%), mental health service referrals for 75 (27%), detoxification for 60 (22%) and referral to Alcoholics Anonymous for 41 (15%). There were 36 (15%) patients who received no medical/supportive interventions, with significantly higher proportions in patients with surgical alcohol-related disease and pregnancy-related conditions (P < 0.05). Conclusion: Bwindi Community Hospital has implemented a program for AUD in three departments, with most individuals screened and managed in the adult in-patient department. While a variety of interventions were given to those with AUD, 15% received no intervention and this deficiency must be addressed. Program performance could improve through better screening processes, ensuring that 100% of those with AUD receive a medical/supportive intervention and raising public awareness.

['Kuule|Yusufu|Y|', 'Dobson|Andrew E|AE|', 'Harries|Anthony D|AD|', 'Mutahunga|Birungi|B|', 'Stewart|Alex G|AG|', 'Wilkinson|Ewan|E|']

2018

25531048

Patient-centered care in cancer treatment programs: the future of integrative oncology through psychoeducation.

The reciprocal relationship between the mind and body has been a neglected process for improving the psychosocial care of cancer patients. Emotions form an important link between the mind and body. They play a fundamental role in the cognitive functions of decision-making and symptom control. Recognizing this relationship is important for integrative oncology. We define psychoeducation as the teaching of self-evaluation and self-regulation of the mind-body process. A gap exists between research evidence and implementation into clinical practice. The patients' search for self-empowerment through the pursuit of complementary therapies may be a surrogate for inadequate psychoeducation. Integrative oncology programs should implement psychoeducation that helps patients to improve both emotional and cognitive intelligence, enabling them to better negotiate cancer treatment systems.

['Garchinski|Christina M|CM|', 'DiBiase|Ann-Marie|AM|', 'Wong|Raimond K|RK|', 'Sagar|Stephen M|SM|']

2014

34599832

The association of interferon-alpha with development of collateral circulation after artery occlusion.

BACKGROUND Previous studies have demonstrated that interferon (IFN) signaling is enhanced in patients with poor collateral circulation (CC). However, the role and mechanisms of IFN-alpha in the development of CC remain unknown. METHODS We studied the serum levels of IFN-alpha and coronary CC in a case-control study using logistics regression, including 114 coronary chronic total occlusion (CTO) patients with good coronary CC and 94 CTO patients with poor coronary CC. Restricted cubic splines was used to flexibly model the association of the levels of IFN-alpha with the incidence of good CC perfusion restoration after systemic treatment with IFN-alpha was assessed in a mice hind-limb ischemia model. RESULTS Compared with the first IFN-alpha tertile, the risk of poor CC was higher in the third IFN-alpha tertile (OR: 4.79, 95% CI: 2.22-10.4, p < .001). A cubic spline-smoothing curve showed that the risk of poor CC increased with increasing levels of serum IFN-alpha. IFN-alpha inhibited the development of CC in a hindlimb ischemia model. Arterioles of CC in the IFN-alpha group were smaller in diameter than in the control group. CONCLUSION Patients with CTO and with poor CC have higher serum levels of IFN-alpha than CTO patients with good CC. IFN-alpha might impair the development of CC after artery occlusion.

['Xing|Zhenhua|Z|', 'Wang|Xiaopu|X|', 'Pei|Junyu|J|', 'Zhu|Zhaowei|Z|', 'Tai|Shi|S|', 'Hu|Xinqun|X|https://orcid.org/0000-0003-1430-4833']

2021

25427889

The Role of Oxidative Stress in Diabetic Neuropathy: Generation of Free Radical Species in the Glycation Reaction and Gene Polymorphisms Encoding Antioxidant Enzymes to Genetic Susceptibility to Diabetic Neuropathy in Population of Type I Diabetic Patients.

Diabetic neuropathy (DN) represents the main cause of morbidity and mortality among diabetic patients. Clinical data support the conclusion that the severity of DN is related to the frequency and duration of hyperglycemic periods. The presented experimental and clinical evidences propose that changes in cellular function resulting in oxidative stress act as a leading factor in the development and progression of DN. Hyperglycemia- and dyslipidemia-driven oxidative stress is a major contributor, enhanced by advanced glycation end product (AGE) formation and polyol pathway activation. There are several polymorphous pathways that lead to oxidative stress in the peripheral nervous system in chronic hyperglycemia. This article demonstrates the origin of oxidative stress derived from glycation reactions and genetic variations within the antioxidant genes which could be implicated in the pathogenesis of DN. In the diabetic state, unchecked superoxide accumulation and resultant increases in polyol pathway activity, AGEs accumulation, protein kinase C activity, and hexosamine flux trigger a feed-forward system of progressive cellular dysfunction. In nerve, this confluence of metabolic and vascular disturbances leads to impaired neural function and loss of neurotrophic support, and over the long term, can mediate apoptosis of neurons and Schwann cells, the glial cells of the peripheral nervous system. In this article, we consider AGE-mediated reactive oxygen species (ROS) generation as a pathogenesis factor in the development of DN. It is likely that oxidative modification of proteins and other biomolecules might be the consequence of local generation of superoxide on the interaction of the residues of L-lysine (and probably other amino acids) with α-ketoaldehydes. This phenomenon of non-enzymatic superoxide generation might be an element of autocatalytic intensification of pathophysiological action of carbonyl stress. Glyoxal and methylglyoxal formed during metabolic pathway are detoxified by the glyoxalase system with reduced glutathione as co-factor. The concentration of reduced glutathione may be decreased by oxidative stress and by decreased in situ glutathione reductase activity in diabetes mellitus. Genetic variations within the antioxidant genes therefore could be implicated in the pathogenesis of DN. In this work, the supporting data about the association between the -262T > C polymorphism of the catalase (CAT) gene and DN were shown. The -262TT genotype of the CAT gene was significantly associated with higher erythrocyte catalase activity in blood of DN patients compared to the -262CC genotype (17.8 ± 2.7 × 10(4) IU/g Hb vs. 13.5 ± 3.2 × 10(4) IU/g Hb, P = 0.0022). The role of these factors in the development of diabetic complications and the prospective prevention of DN by supplementation in formulations of transglycating imidazole-containing peptide-based antioxidants (non-hydrolyzed carnosine, carcinine, n-acetylcarcinine) scavenging ROS in the glycation reaction, modifying the activity of enzymic and non-enzymic antioxidant defenses that participate in metabolic processes with ability of controlling at transcriptional levels the differential expression of several genes encoding antioxidant enzymes inherent to DN in Type I Diabetic patients, now deserve investigation.

['Babizhayev|Mark A|MA|', 'Strokov|Igor A|IA|', 'Nosikov|Valery V|VV|', "Savel'yeva|Ekaterina L|EL|", 'Sitnikov|Vladimir F|VF|', 'Yegorov|Yegor E|YE|', 'Lankin|Vadim Z|VZ|']

2015

32255965

Dynamic evolution of canine parvovirus in Thailand.

Background and Aim According to the previous study, the circulating canine parvovirus (CPV) in Thailand is 2a and 2b. Nowadays, CPV mutants, including CPV-2c, have been identified in many parts of the world. This study aimed to investigate the genetic diversity of the circulating CPV in Thailand. Materials and Methods Eighty-five CPV-positive fecal samples were obtained from dogs with either acute hemorrhagic diarrhea or diarrhea. The complete VP2 gene of these samples was amplified using VP2 specific primers and polymerase chain reaction (PCR). The obtained full-length VP2 sequences were analyzed and a phylogenetic tree was constructed. Results Sixty and 25 CPV-positive fecal samples were collected in 2010 and 2018, respectively. Thirty-four samples were new CPV-2a and 31 samples were new CPV-2b due to amino acids substitution at position 297 (Ser-Ala). In 2018, 5 new CPV-2a, 19 CPV-2c, and 1 feline panleukopenia virus (FPV) were found, but no new CPV-2b was detected. Moreover, most of the CPV in this study had amino acids mutations at positions 324 and 440. The phylogenetic construction demonstrated the close relationship between the current new CPV-2a with the previous CPV-2a reported from Thailand, China, Uruguay, Vietnam, Singapore, and India. Interestingly, the current new CPV-2b in this study was not closely related to the previous CPV-2b reported in Thailand. The CPV-2c in this study was closer to Asian CPV-2c and further from either European or South America CPV-2c. Interestingly, FPV was identified in a diarrhea dog. Conclusion The evolution of CPV in Thailand is very dynamic. Thus, it is important to monitor for CPV mutants and especially the clinical signs relating to these mutants to conduct surveillance for the emergence of new highly pathogenic CPV in the future.

['Inthong|N|N|', 'Kaewmongkol|S|S|', 'Meekhanon|N|N|', 'Sirinarumitr|K|K|', 'Sirinarumitr|T|T|']

2020

28269813

Evaluation of noise limits to improve image processing in soft X-ray projection microscopy.

Soft X-ray microscopy has been developed for high resolution imaging of hydrated biological specimens due to the availability of water window region. In particular, a projection type microscopy has advantages in wide viewing area, easy zooming function and easy extensibility to computed tomography (CT). The blur of projection image due to the Fresnel diffraction of X-rays, which eventually reduces spatial resolution, could be corrected by an iteration procedure, i.e., repetition of Fresnel and inverse Fresnel transformations. However, it was found that the correction is not enough to be effective for all images, especially for images with low contrast. In order to improve the effectiveness of image correction by computer processing, we in this study evaluated the influence of background noise in the iteration procedure through a simulation study. In the study, images of model specimen with known morphology were used as a substitute for the chromosome images, one of the targets of our microscope. Under the condition that artificial noise was distributed on the images randomly, we introduced two different parameters to evaluate noise effects according to each situation where the iteration procedure was not successful, and proposed an upper limit of the noise within which the effective iteration procedure for the chromosome images was possible. The study indicated that applying the new simulation and noise evaluation method was useful for image processing where background noises cannot be ignored compared with specimen images.

['Jamsranjav|Erdenetogtokh|E|', 'Kuge|Kenichi|K|', 'Ito|Atsushi|A|', 'Kinjo|Yasuhito|Y|', 'Shiina|Tatsuo|T|']

2017

23896686

A high-throughput screening assay for fungicidal compounds against Cryptococcus neoformans.

Cryptococcus neoformans is a pathogenic fungus that causes meningitis worldwide, particularly in human immunodeficiency virus (HIV)-infected individuals. Although amphotericin B is the "gold standard" treatment for cryptococcal meningitis, the toxicity and inconvenience of intravenous injection emphasize a need for development of new anticryptocccal drugs. Recent data from humans and animal studies suggested that a nutrient-deprived host environment may exist in cryptococcal meningitis. Thus, a screening assay for identifying fungicidal compounds under nutrient-deprived conditions may provide an alternative strategy to develop new anticryptococcal drugs for this disease. A high-throughput fungicidal assay was developed using a profluorescent dye, alamarBlue, to detect residual metabolic activity of C. neoformans under nutrient-limiting conditions. Screening the Library of Pharmacologically Active Compounds (LOPAC) with this assay identified a potential chemical scaffold, 10058-F4, that exhibited fungicidal activity in the low micromolar range. These results thus demonstrate the feasibility of this alamarBlue-based assay for high-throughput screening of fungicidal compounds under nutrient-limiting conditions for new anticryptococcal drug development.

['Rabjohns|Jennifer L A|JLA|', 'Park|Yoon-Dong|YD|', 'Dehdashti|Jean|J|', 'Henderson|Christina|C|', 'Zelazny|Adrian|A|', 'Metallo|Steven J|SJ|', 'Zheng|Wei|W|', 'Williamson|Peter R|PR|']

2014

26223261

Resting-State Functional Connectivity of the Locus Coeruleus in Humans: In Comparison with the Ventral Tegmental Area/Substantia Nigra Pars Compacta and the Effects of Age.

The locus coeruleus (LC) provides the primary noradrenergic inputs to the cerebral cortex. Despite numerous animal studies documenting the functions of the LC, research in humans is hampered by the small volume of this midbrain nucleus. Here, we took advantage of a probabilistic template, explored the cerebral functional connectivity of the LC with resting-state fMRI data of 250 healthy adults, and verified the findings by accounting for physiological noise in another data set. In addition, we contrasted connectivities of the LC and the ventral tegmental area/substantia nigra pars compacta. The results highlighted both shared and distinct connectivity of these 2 midbrain structures, as well as an opposite pattern of connectivity to bilateral amygdala, pulvinar, and right anterior insula. Additionally, LC connectivity to the fronto-parietal cortex and the cerebellum increases with age and connectivity to the visual cortex decreases with age. These findings may facilitate studies of the role of the LC in arousal, saliency responses and cognitive motor control and in the behavioral and cognitive manifestations during healthy and disordered aging. Although the first to demonstrate whole-brain LC connectivity, these findings need to be confirmed with high-resolution imaging.

['Zhang|Sheng|S|', 'Hu|Sien|S|', 'Chao|Herta H|HH|', 'Li|Chiang-Shan R|CS|']

2016

32909274

Is gene panel sequencing more efficient than clinical-based gene sequencing to diagnose autoinflammatory diseases? A randomized study.

The aim of this study was to compare the effectiveness of the gene-panel next-generation sequencing (NGS) strategy versus the clinical-based gene Sanger sequencing for the genetic diagnosis of autoinflammatory diseases (AIDs). Secondary goals were to describe the gene and mutation distribution in AID patients and to evaluate the impact of the genetic report on the patient's medical care and treatment. Patients with AID symptoms were enrolled prospectively and randomized to two arms, NGS (n = 99) (32-55 genes) and Sanger sequencing (n = 197) (one to four genes). Genotypes were classified as 'consistent/confirmatory', 'uncertain significance' or 'non-contributory'. The proportion of patients with pathogenic genotypes concordant with the AID phenotype (consistent/confirmatory) was significantly higher with NGS than Sanger sequencing [10 of 99 (10·1%) versus eight of 197 (4·1%)]. MEFV, ADA2 and MVK were the most represented genes with a consistent/confirmed genotype, whereas MEFV, NLRP3, NOD2 and TNFRSF1A were found in the 'uncertain significance' genotypes. Six months after the genetic report was sent, 54 of 128 (42·2%) patients had received effective treatment for their symptoms; 13 of 128 (10·2%) had started treatment after the genetic study. For 59 of 128 (46%) patients, the results had an impact on their overall care, independent of sequencing group and diagnostic conclusion. Targeted NGS improved the diagnosis and global care of patients with AIDs.

['Rama|M|M|', 'Mura|T|T|', 'Kone-Paut|I|I|', 'Boursier|G|G|', 'Aouinti|S|S|', 'Touitou|I|I|', 'Sarrabay|G|G|0000-0002-9943-9368']

2021

32342559

Efficacy and safety of a soft contact lens to control myopia progression.

CLINICAL RELEVANCE The control of myopia progression is currently considered an evidence-based therapeutic need. BACKGROUND To determine the efficacy and safety of the Esencia lens, a new soft contact lens (SCL) designed to slow down myopia progression in paediatric patients. METHODS This study was a randomised, parallel, double-masked clinical trial. Seventy myopic (-0.50 to -8.75 D) boys and girls, 7-15-years of age, were randomised and allocated to one of two groups: (i) study (n = 36) or (ii) control (n = 34). Study group patients were given the Esencia lens, a progressive multifocal and reverse geometry SCL. Control group patients were given conventional SCLs. Efficacy measurements (change in cycloplegic autorefraction and axial length) were measured at baseline and at the six-month intervals over a 12- month period. Visual performance measurements were corneal power, comfort, quality of vision and contact lens fitting. Safety measures included detection of adverse events. RESULTS Mean changes in cycloplegic autorefraction after 12-months were -0.28 ± 0.35 D for study and -0.57 ± 0.52 D for control group patients (p = 0.02). A significantly lower increase in axial length was found in the study group (0.13 ± 0.12-mm) compared to control (0.22 ± 0.14-mm) patients (p = 0.03). Compared to control group patients, there was less myopia progression in the study group: 51 and 41 per cent in terms of cycloplegic autorefraction and axial length, respectively. No significant differences between groups for change in corneal power, comfort, vision quality and contact lens fitting were found (p > 0.05). Regarding safety, there were no serious and/or unexpected adverse events during the study. CONCLUSIONS The Esencia lens seems to be efficacious in slowing down progression of myopia in children compared to traditional SCLs in the short term, with comparable safety features and visual outcomes.

['Garcia-Del Valle|Alba M|AM|', 'Blázquez|Vanesa|V|', 'Gros-Otero|Juan|J|', 'Infante|Mónica|M|', 'Culebras|Antonio|A|', 'Verdejo|Antonio|A|', 'Sebastián|Javier|J|', 'García|Marcos|M|', 'Bueno|Sara|S|', 'Piñero|David P|DP|']

2021

32940564

The Effects of Acute and Chronic Exercise on Paraoxonase-1 (PON1): A Systematic Review With Meta-Analysis.

Purpose: To determine the acute and chronic effects of exercise on Paraoxonase-1 (PON1) concentration and activity. Methods: A literature search was performed using 16 electronic databases. Effect sizes (ES) were computed and two-tailed α values < .05 and non-overlapping 95% confidence intervals (95%CI) were considered statistically significant. Heterogeneity, inconsistency (I), and small-study effects using the LFK index were examined. Results: Eighteen studies (n = 377 participants) met the criteria for inclusion. The acute effects of exercise on PON1 concentration were trivial and non-significant (ES = -.03, 95%CI = -.39 to .34, p > .05), heterogeneous (p = .05), moderately inconsistent (I = 48%), with minor asymmetry (LFK index = 1.34). The chronic effects of exercise on PON1 concentration were also trivial and non-significant (ES = -.04, 95%CI = -.53 to.45, p > .05), homogenous (p = .65), displayed low inconsistency (I = 0%), and minor asymmetry (LFK index = -1.14). The acute effects of exercise on PON1 activity were trivial and non-significant (ES = .11, 95%CI = -.02 to.24, p > .05), homogenous (p = .85), showed low inconsistency (I = 0%), and no asymmetry (LFK index = .82). The chronic effects of exercise on PON1 activity were trivial and non-significant (ES = .31, 95%CI = -.03 to.65, p > .05), homogenous (p = .17), moderately inconsistent (I2 = 36%), with no asymmetry (LFK index = .60). Conclusion: Acute and chronic exercise training, overall, exerted a trivial effect on PON1 concentration and activity.

['Taylor|James Kyle|JK|https://orcid.org/0000-0001-6688-1718', 'Carpio-Rivera|Elizabeth|E|https://orcid.org/0000-0002-4490-4517', 'Chacón-Araya|Yamileth|Y|https://orcid.org/0000-0001-8070-1111', 'Grandjean|Peter W|PW|https://orcid.org/0000-0001-9932-6278', 'Moncada-Jiménez|José|J|https://orcid.org/0000-0001-9807-5163']

2020

28097068

Hepatic fibrosis and factors associated with liver stiffness in HIV mono-infected individuals.

BACKGROUND Liver disease has become an important cause of morbidity and mortality even in those HIV-infected individuals who are devoid of hepatitis virus co-infection. The aim of this study was to evaluate the degree of hepatic fibrosis and the role of associated factors using liver stiffness measurement in HIV mono-infected patients without significant alcohol intake. METHODS We performed a cross-sectional study of 101 HIV mono-infected patients recruited prospectively from March 1, 2014 to October 30, 2014 at the Center for HIV, St István and St László Hospital, Budapest, Hungary. To determine hepatic fibrosis, liver stiffness was measured with transient elastography. Demographic, immunologic and other clinical parameters were collected to establish a multivariate model. Bayesian Model Averaging (BMA) was performed to identify predictors of liver stiffness. RESULTS Liver stiffness ranged from 3.0-34.3 kPa, with a median value of 5.1 kPa (IQR 1.7). BMA provided a very high support for age (Posterior Effect Probability-PEP: 84.5%), moderate for BMI (PEP: 49.3%), CD4/8 ratio (PEP: 44.2%) and lipodystrophy (PEP: 44.0%). For all remaining variables, the model rather provides evidence against their effect. These results overall suggest that age and BMI have a positive association with LS, while CD4/8 ratio and lipodystrophy are negatively associated. DISCUSSION Our findings shed light on the possible importance of ageing, overweight and HIV-induced immune dysregulation in the development of liver fibrosis in the HIV-infected population. Nonetheless, further controlled studies are warranted to clarify causal relations.

['Sulyok|Mihály|M|', 'Ferenci|Tamás|T|', 'Makara|Mihály|M|', 'Horváth|Gábor|G|', 'Szlávik|János|J|', 'Rupnik|Zsófia|Z|', 'Kormos|Luca|L|', 'Gerlei|Zsuzsanna|Z|', 'Sulyok|Zita|Z|', 'Vályi-Nagy|István|I|']

2017

29761429

Guidelines for Sample Normalization to Minimize Batch Variation for Large-Scale Metabolic Profiling of Plant Natural Genetic Variance.

Recent methodological advances in both liquid chromatography-mass spectrometry (LC-MS) and gas chromatography-mass spectrometry (GC-MS) have facilitated the profiling highly complex mixtures of primary and secondary metabolites in order to investigate a diverse range of biological questions. These techniques usually face a large number of potential sources of technical and biological variation. In this chapter we describe guidelines and normalization procedures to reduce the analytical variation, which are essential for the high-throughput evaluation of metabolic variance used in broad genetic populations which commonly entail the evaluation of hundreds or thousands of samples. This chapter specifically deals with handling of large-scale plant samples for metabolomics analysis of quantitative trait loci (mQTL) in order to reduce analytical error as well as batch-to-batch variation.

['Alseekh|Saleh|S|', 'Wu|Si|S|', 'Brotman|Yariv|Y|', 'Fernie|Alisdair R|AR|']

2018

34095509

Feasibility and validation of trans-valvular flow derived by four-dimensional flow cardiovascular magnetic resonance imaging in patients with atrial fibrillation.

Background: Four-dimensional (4D) flow cardiovascular magnetic resonance imaging (MRI) is an emerging technique used for intra-cardiac blood flow assessment. The role of 4D flow cardiovascular MRI in the assessment of trans-valvular flow in patients with atrial fibrillation (AF) has not previously been assessed. The purpose of this study was to assess the feasibility, image quality, and internal validity of 4D flow cardiovascular MRI in the quantification of trans-valvular flow in patients with AF. Methods: Patients with AF and healthy controls in sinus rhythm underwent cardiovascular MRI, including 4D flow studies. Quality assurance checks were done on the raw data and streamlines. Consistency was investigated by trans-valvular flow assessment between the mitral valve (MV) and the aortic valve (AV). Results: Eight patients with AF (88% male, mean age 62±13 years, mean heart rate (HR) 83±16 beats per minute (bpm)) were included and compared with ten healthy controls (70% male, mean age 41±20 years, mean HR 68.5±9 bpm). All scans were of either good quality with minimal blurring artefacts, or excellent quality with no artefacts. No significant bias was observed between the AV and MV stroke volumes in either healthy controls (-4.8, 95% CI -15.64 to 6.04; P=0.34) or in patients with AF (1.64, 95% CI -4.7 to 7.94; P=0.56). A significant correlation was demonstrated between MV and AV stroke volumes in both healthy controls (r=0.87, 95% CI 0.52 to 0.97; P=0.001) and in AF patients (r=0.82, 95% CI 0.26 to 0.97; P=0.01). Conclusions: In patients with AF, 4D flow cardiovascular MRI is feasible with good image quality, allowing for quantification of trans-valvular flow.

['Mills|Mark T|MT|https://orcid.org/0000-0002-5425-7739', 'Grafton-Clarke|Ciaran|C|https://orcid.org/0000-0002-8537-0806', 'Williams|Gareth|G|https://orcid.org/0000-0002-7356-3889', 'Gosling|Rebecca C|RC|', 'Al Baraikan|Abdulaziz|A|https://orcid.org/0000-0002-7889-4476', 'Kyriacou|Andreas L|AL|', 'Morris|Paul D|PD|https://orcid.org/0000-0002-3965-121X', 'Gunn|Julian P|JP|https://orcid.org/0000-0003-0028-3226', 'Swoboda|Peter P|PP|https://orcid.org/0000-0001-7162-7079', 'Levelt|Eylem|E|', 'Tsampasian|Vasiliki|V|https://orcid.org/0000-0003-1534-7587', 'van der Geest|Rob J|RJ|', 'Swift|Andrew J|AJ|', 'Greenwood|John P|JP|https://orcid.org/0000-0002-2861-0914', 'Plein|Sven|S|', 'Vassiliou|Vass|V|https://orcid.org/0000-0002-4005-7752', 'Garg|Pankaj|P|https://orcid.org/0000-0002-5483-169X']

2021

27287038

Evidence that ghrelin may be associated with the food intake of gibel carp (Carassius auratus gibelio).

Ghrelin, a non-amidated peptide hormone, is a potent anorectic neuropeptide implicated in feeding regulation in mammals and non-mammalian vertebrates. However, the involvement of ghrelin in the feeding behavior of teleosts has not been well understood. To better understand the role of ghrelin in the regulation of appetite in fish, in this study, we cloned the cDNAs encoding ghrelin and investigated their mRNA distributions in gibel carp tissues. We also assessed the effects of different nutritional status on ghrelin mRNA abundance. Ghrelin mRNAs were ubiquitously expressed in ten tissues (intestine, liver, brain, mesonephron, head kidney, spleen, skin, heart, muscle, gill and pituitary gland), and relatively high expression levels were detected in the gut. Postprandial studies analysis revealed a significant postprandial decrease in ghrelin mRNA expression in the gut (1 and 3 h after the regular feeding time). In addition, ghrelin mRNA expression in the gut significantly increased at day 7 after fasting and declined sharply after refeeding, which suggested that ghrelin might be involved in the regulation of appetite in gibel carp. Overall, our result provides basis for further investigation into the regulation of feeding in gibel carp.

['Zhou|Chaowei|C|', 'Zheng|Jinfa|J|', 'Lei|Luo|L|', 'Yuan|Dengyue|D|', 'Zhu|Chengke|C|', 'Ye|Hua|H|', 'Zhang|Chi|C|', 'Wang|Dan|D|', 'Yang|Minmin|M|', 'Wu|Jingjing|J|', 'Zhu|Long|L|', 'Zeng|Benhe|B|']

2016

33881965

Differentially expressed genes PCCA, ECHS1, and HADH are potential prognostic biomarkers for gastric cancer.

Gastric cancer (GC) is one of the most common malignant tumors in the world. As far as we know, no biomarker has been widely accepted for early diagnosis and prognosis prediction of GC. The purpose of this study is to find potential biomarkers to predict the prognosis of GC. The differentially expressed gene (DEG) was analyzed from GSE93774. Enrichr was used to analyze the gene ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway, the enrichment of transcription factors (TF), miRNA, and kinase. GO analysis showed DEGs was enriched in the process of amino acid metabolism. Pathway results showed DEGs was mainly enriched in cell cycle. Propionyl CoA carboxylase alpha (PCCA), Enoyl coenzyme A hydratase short chain 1 (ECHS1), and 3-hydroxyacyl-CoA dehydrogenase (HADH) have prognostic value in patients with GC. ECHS1 and HADH genes were significantly associated with disease-free survival. There was a significant correlation between PCCA and overall survival rate. The results of this study suggest that PCCA, ECHS1, and HADH may be new biomarkers for predicting the prognosis of GC.

['Du|Zhongxiang|Z|', 'Zhang|Xiajun|X|', 'Gao|Weiya|W|', 'Yang|Jie|J|https://orcid.org/0000-0002-8524-2580']

2021

24295123

Attentional control, attentional network functioning, and emotion regulation styles.

Attentional network functioning in emotionally neutral conditions and self-reported attentional control (AC) were analysed as predictors of the tendency to engage in dysfunctional emotion regulation strategies. Diminished attentional orienting predicted an increased tendency to engage in brooding rumination, and enhanced alertness predicted a greater chance of suppression, beyond trait anxiety and self-reported AC, which were not predictive of either rumination or suppression. This is the first study to show that some forms of dysfunctional emotion regulation are related to the attentional network functioning in emotionally neutral conditions. Results are discussed in relation to regulatory temperament and anxiety-related attentional biases literature.

['Tortella-Feliu|Miquel|M|', 'Morillas-Romero|Alfonso|A|', 'Balle|Maria|M|', 'Bornas|Xavier|X|', 'Llabrés|Jordi|J|', 'Pacheco-Unguetti|Antonia P|AP|']

2014

33034537

Unassisted N-acetyl-phenylalanine-amide transport across membrane with varying lipid size and composition: kinetic measurements and atomistic molecular dynamics simulation.

Biological membranes are essential to preserve structural integrity and regulate functional properties through the permeability of nutrients, pharmaceutical drugs, and neurotransmitters of a living cell. The movement of acetylated and amidated phenylalanine (NAFA) across 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) membrane bilayers is investigated to probe physical transport. The rate of transport is measured experimentally applying parallel artificial membrane permeation assay (PAMPA). At the physiological temperature, 310 K, the measured time constants in the neutral pH were ∼6 h in DOPC and ∼3 h in POPC, while in a more acidic condition, at a pH 4.8, the time constants were ∼8 h in both lipids. Computationally, we have expanded our transport study of three aromatic dipeptides across a bilayer composed of DOPC18. In this study, we have examined the effects of lipid composition and bilayer size on the passive transport of NAFA by simulating the dipeptide in three different bilayers, with 50 DOPC lipids, 50 POPC lipids, and 40 POPC molecules. Specifically, atomistic molecular dynamics simulations with umbrella sampling were used to calculate the potential of mean force for the passive permeation of NAFA across the bilayers. Diffusion constants were then calculated by numerically solving the Smoluchowski equation. Permeability coefficients and mean first passage times were then calculated. Structural properties - Ramachandran plots, sidechain torsions, peptide insertion angles, radial distribution functions, and proximal peptide water molecules - were also examined to determine the effect of system size and lipid type. In terms of systems size, we observed a small decrease in the highest barrier of the potential of mean force and fewer sampled sidechain dihedral angle conformations with 40 versus 50 POPC lipids due to weaker membrane deformations within a smaller lipid bilayer. In terms of lipid type, DOPC contains two monounsaturated acyl chains compared to only one such acyl chain in POPC; therefore, DOPC bilayers are less ordered and more easily deformed, as seen by a much broader potential of mean force profile. The NAFA in DOPC lipid also transitioned to an internally hydrogen-bonded backbone conformation at lower membrane depths than in POPC. Similarly, as for other aromatic dipeptides, NAFA tends to insert into the membrane sidechain-first, remains mostly desolvated in the membrane center, and exhibits slow reorientations within the bilayer in both DOPC and POPC. With a joint experimental and computational study we have gained a new insight into the rate of transport and the underlying microscopic mechanism in different lipid bilayer conditions of the simplest hydrophobic aromatic dipeptide.Communicated by Ramaswamy H. Sarma.

['Lee|Brent L|BL|', 'Kuczera|Krzysztof|K|', 'Lee|Kyung-Hoon|KH|', 'Childs|Ed W|EW|', 'Jas|Gouri S|GS|https://orcid.org/0000-0002-0774-012X']

2020

25105263

Study on the interaction of Co (III) DiAmsar with serum albumins: spectroscopic and molecular docking methods.

This study was designed to examine the interaction of cobalt-3,6,10,13,16,19-hexaazabicyclo[6.6.6]eicosane-1,8-diamine (Co(III) DiAmsar) as a hexadentate ligand with human serum albumin (HSA) and bovine serum albumin (BSA) under physiological conditions in Tris-HCl buffer solution at pH 7.4. To this aim, at first, Co (III) DiAmsar was synthesized and characterized by nuclear magnetic resonance (NMR), and mass spectroscopy and then its interaction with HSA and BSA was investigated by means of various spectroscopic methods (Fourier transform infrared (FT-IR), UV-visible (UV-vis), fluorescence, and cyclic voltammetry (CV)) and molecular docking technique. The results of fluorescence titration revealed that the Co (III) DiAmsar strongly quench the intrinsic fluorescence of HSA and BSA through a static quenching procedure. Binding constants (Ka) and the number of binding sites (n∼1) were calculated using Stern-Volmer equations. The ΔG parameters at different temperatures were calculated. Subsequently, the values of ΔH and ΔS were also calculated, which revealed that the van der Waals and hydrogen bonding interaction splay a major role in Co (III) DiAmsar-HSA and Co (III) DiAmsar-BSA associations. The distance r between donor (HSA and BSA) and acceptor (Co (III) DiAmsar) was obtained according to fluorescence resonance energy transfer. The data obtained by the molecular modeling study revealed the surrounding residues of HSA and BSA around Co (III) DiAmsar.

['Farahani|Bahman Vasheghani|BV|', 'Bardajee|Ghasem Rezanejade|GR|', 'Rajabi|Farzaneh Hosseinpour|FH|', 'Hooshyar|Zari|Z|']

2015

28285256

Interleukin-6 and depressive symptom severity in response to physical exercise.

Elevated IL-6 has been implicated in depression. The anti-inflammatory effects of exercise may be associated with its clinical efficacy for depression. We determined if serum IL-6 levels were altered by 12 weeks of physical exercise, and if IL-6 levels were associated with baseline depression severity and change in depression severity in response to exercise. Data from 116 adults (42.7±11.5y) with mild-to-moderate depression (Patient Health Questionnaire >9) who participated in the physical exercise arm of the Regassa RCT (www.regassa.se) were analyzed. Participants were requested to complete three 60-min exercise sessions weekly for 12 weeks. Blood samples were provided at baseline and post-intervention following an overnight fast and were analyzed for serum levels of IL-6 using ELISA. IL-6 values were logarithm-transformed. Higher baseline serum IL-6 levels were significantly associated with reduced depression severity after exercise. Reduced IL-6 levels following exercise were significantly associated with parallel reductions in depression severity. These findings are consistent with a previously reported association between reduced serum IL-1β levels and reduced depression severity following 12 weeks of physical exercise in 105 depressed adults. Findings support associations between IL-6, depressive symptoms, and exercise response, and provide support for the plausible involvement of IL-6 in the antidepressive effect of exercise.

['Lavebratt|Catharina|C|', 'Herring|Matthew P|MP|', 'Liu|Jia Jia|JJ|', 'Wei|Ya Bin|YB|', 'Bossoli|Davide|D|', 'Hallgren|Mats|M|', 'Forsell|Yvonne|Y|']

2017

28322630

Energy, macronutrient and water intake during a mountain ultramarathon event: The influence of distance.

Adequate dietary strategies are essential for the successful participation in ultra-endurance races. The aim of this study was to evaluate and compare the energy and water intakes of participants during three different mountain ultra-endurance runs. The study took place at the "Ultra Mallorca Serra de Tramuntana" (Mallorca, Spain), an ultra-endurance mountain event with runners participating either in a 44-km (Marathon, n = 51), a 67-km (Trail, n = 109) or a 112-km (Ultra, n = 53) run competition. Participants in the study answered a questionnaire focused on the nutritional intake within an hour after finishing the competition. Mean energy intake during the competitions was 183 kcal · h¯1, with an average carbohydrate intake of 31 g · h¯1 (52.1% of participants consumed less than 30 g · h¯1). No significant differences between competitions were found in these parameters. However, a higher percentage of energy from lipids in participants in the Trail and the Ultra was found (P = 0.034). Furthermore, significant differences were observed in water intake per hour of competition (P = 0.039), with the lowest value for the intake during the Ultra competition. In conclusion, the majority of the participants in the study present low carbohydrate intakes. However, fluid intake seems to be adequate. Different distances did not significantly influence the participants' nutritional strategies.

['Martinez|Sonia|S|', 'Aguilo|Antoni|A|', 'Rodas|Lluis|L|', 'Lozano|Leticia|L|', 'Moreno|Carlos|C|', 'Tauler|Pedro|P|']

2018

24269201

Cell signaling mechanisms of oro-gustatory detection of dietary fat: advances and challenges.

CD36 and two G-protein coupled receptors (GPCR), i.e., GPR120 and GPR40, have been implicated in the gustatory perception of dietary fats in rodents. These glycoproteins are coupled to increases in free intracellular Ca²⁺ concentrations, [Ca²⁺](i), during their activation by dietary long-chain fatty acids (LCFA). The transient receptor potential type M5 (TRPM5) channel, activated by [Ca²⁺](i), participates in downstream signaling in taste bud cells (TBC). The mice, knocked-out for expression of CD36, GPR120, GPR40 or TRPM5 have a reduced spontaneous preference for fat. The delayed rectifying K⁺ (DRK) channels believed to lie downstream of these receptors are also important players in fat taste transduction. The trigeminal neurons by triggering increases in [Ca²⁺](i) may influence the taste signal to afferent nerve fibers. Why are there so many taste receptor candidates for one taste modality? We discuss the recent advances on the role of CD36, GPR120, GPR40, TRPM5 and DRK channels, in signal transduction in TBC. We shed light on their cross-talk and delineate their roles in obesity as a better understanding of the molecular mechanisms behind their regulation could eventually lead to new strategies to fight against this condition.

['Gilbertson|Timothy A|TA|', 'Khan|Naim A|NA|']

2014

33940728

The effect of the European Industrial Emissions Directive on the air emission limit values set by competent authorities in the permitting procedure: The case of the Spanish cement industry.

The concept of the "flexibility principle" introduced by the Integrated Pollution Prevention and Control Directive in the procedure to determine the emission limit values in the permit raised the interest of several scholars and heightened the debate on environmental regulation. The Integrated Pollution Prevention and Control Directive allows a considerable flexibility to competent authorities in the permitting process to deviate from the use of best available techniques described in the reference documents. Several studies show how this flexibility lead to disparities in the permitting process among Member States, which reduced the potential benefits in the environmental performance of companies. After the adoption of the Industrial Emissions Directive by the Member States of the European Union, the permit contents of around 52,000 of the largest European Union industrial installations need to be updated by competent authorities to meet the requirements of this Directive. Several studies on its effectiveness have been developed to determine how emission limit values are set by competent authorities with reference to the range of emission levels associated with the best available techniques. This paper is oriented to study the effect of the Industrial Emissions Directive at sector level, investigating its effects on the air emission limit values set by the competent authorities in the permits for cement facilities. This paper contributes to the scientific debate in relation to the effect of these Directives on the environmental performance of the industrial installations. The data analysed in the case study considered show that the emission limit values have been reduced in permits consistently over the past 10 years.

['Calvo|Víctor Luis Vázquez|VLV|', 'Giner-Santonja|Germán|G|', 'Alonso-Fariñas|Bernabé|B|', 'Aguado|José Morillo|JM|']

2021

26017337

Metaethnographic Synthesis of Fathers' Experiences of the Neonatal Intensive Care Unit Environment During Hospitalization of Their Premature Infants.

OBJECTIVE To synthesize existing qualitative findings about fathers' experiences of the neonatal intensive care unit (NICU) environment. DATA SOURCES Relevant key terms including preterm, father, and NICU were used to search the databases of CINAHL Plus, Academic Search Premier, MEDLINE, and PsychInfo. STUDY SELECTION Only primary qualitative studies were included. Studies were excluded that did not focus on the NICU environment. DATA EXTRACTION Twenty-four studies were included. All authors critically appraised and extracted data relating to fathers' experiences in the NICU using an agreed data extraction form. DATA SYNTHESIS Findings were synthesized by translating the initial concepts and findings from an identified key paper into the data from the remaining 23 studies. Initially this was done separately by each author followed by further group discussion and synthesis. Emergent themes included Proximity, Parental Autonomy, Vulnerability, Communication and Exclusion, and Isolation. CONCLUSIONS The needs of fathers to interact and be involved with their infants' care was a prominent factor that enhanced their experiences in the NICU. Staff in the NICU can play a key role in facilitating this interaction through encouragement and reassurance.

['Sisson|Helen|H|', 'Jones|Catriona|C|', 'Williams|Rhona|R|', 'Lachanudis|Lisa|L|']

2015

32537648

Tudor domain of histone demethylase KDM4B is a reader of H4K20me3.

The lysine histone demethylase KDM4B is overexpressed in several types of cancers and plays dual roles in genome stability maintenance. Although KDM4B is able to recognize several histone methylations, the underlying molecular mechanism is still unknown. In this study, we purified the KDM4B chromatin-associated hybrid tudor domains (HTDs) and plant home domains (PHDs) and performed the pull-down assay to screen the tri-methyl modified histone peptides that could be efficiently recognized by KDM4B. Our results showed that both HTD alone and the combination of HTD and PHD were able to specifically bind to H3K4me3 and H4K20me3. Because H4K20me3 is essential for KDM4B's rapid recruitment to DNA damage site, we further aligned the multiple tudor peptide sequence and identified two conserved residues Y993 and W987 that are critical for KDM4B-H4K20me3 interaction. The surface plasmon resonance analysis revealed that HTD displayed a rapid H4K20me3 bind-dissociate pattern. These findings therefore provided mechanistic insights into the binding of tudor domain of KDM4B protein with H4K20me3.

['Xiang|Ying|Y|', 'Guo|Jing|J|', 'Li|Feng|F|', 'Xiong|Jie|J|']

2020

24140845

Dual energy computerized tomography with a split bolus-a 1-stop shop for patients with suspected urinary stones?

PURPOSE We evaluated a dual energy, split bolus computerized tomography protocol that provides virtual noncontrast, parenchymal and urographic phases in a single scan. We assessed the sensitivity of the virtual noncontrast phase using this protocol to detect urinary stones compared to the gold standard of the true noncontrast phase. MATERIALS AND METHODS We prospectively enrolled in the study 81 patients who underwent unenhanced single energy computerized tomography at 120 kV/200 mA as well as contrast enhanced dual energy computerized tomography on a Somatom® Definition Flash-CT (tube A 80 kV/233 mA and tube B SN 140 kV/180 mA with 1/0.8 mm slice thickness). For the split bolus protocol 400 mg/ml Iomeron® were injected at 2 time points, that is 15 ml 10 minutes before the scan and 80 ml 65 seconds before the scan. In a consensus reading 2 readers evaluated the presence and diameter of stones on the true and virtual noncontrast phases. RESULTS Of the 350 stones noted on the true noncontrast phase we found 289 on the virtual noncontrast phase as well as 13 false-positive and 66 false-negative stones. Sensitivity was 98.4%, 89.8% and 82.6% per patient, segment and stone, respectively. The diameter measured on the virtual noncontrast phase corresponded to a mean ± SD 92.5% ± 31.6% of the diameter on the true noncontrast phase. The mean effective dose was 4.8 ± 1.8 and 10.5 ± 3.7 mSv for the true and virtual noncontrast phases, respectively. CONCLUSIONS The proposed protocol allows for combining 3 phases in a single scan while still enabling the detection of urinary stones at high sensitivity. This technique halves the radiation dose and provides the surgeon with better anatomical information on the calyceal system. Therefore, it is a valuable diagnostic tool for kidney stone treatment planning and followup.

['Toepker|Michael|M|', 'Kuehas|Franklin|F|', 'Kienzl|Daniela|D|', 'Herwig|Ralf|R|', 'Spazierer|Elisa|E|', 'Krauss|Bernhard|B|', 'Weber|Michael|M|', 'Seitz|Christian|C|', 'Ringl|Helmut|H|']

2014

34101199

Macaque monkey trigeminal blink reflex circuits targeting levator palpebrae superioris motoneurons.

For normal viewing, the eyes are held open by the tonic actions of the levator palpebrae superioris (levator) muscle raising the upper eyelid. This activity is interrupted during blinks, when the eyelid sweeps down to spread the tear film or protect the cornea. We examined the circuit connecting the principal trigeminal nucleus to the levator motoneurons by use of both anterograde and retrograde tracers in macaque monkeys. Injections of anterograde tracer were made into the principal trigeminal nucleus using either a stereotaxic approach or localization following physiological characterization of trigeminal second order neurons. Anterogradely labeled axonal arbors were located both within the caudal central subdivision, which contains levator motoneurons, and in the adjacent supraoculomotor area. Labeled boutons made synaptic contacts on retrogradely labeled levator motoneurons indicating a monosynaptic connection. As the eye is also retracted through the actions of the rectus muscles during a blink, we examined whether these trigeminal injections labeled boutons contacting rectus motoneurons within the oculomotor nucleus. These were not found when the injection sites were confined to the principal trigeminal nucleus region. To identify the source of the projection to the levator motoneurons, we injected retrograde tracer into the oculomotor complex. Retrogradely labeled cells were confined to a narrow, dorsoventrally oriented cell population that lined the rostral edge of the principal trigeminal nucleus. Presumably these cells inhibit levator motoneurons, while other parts of the trigeminal sensory complex are activating orbicularis oculi motoneurons, when a blink is initiated by sensory stimuli contacting the face.

['Warren|Susan|S|', 'May|Paul J|PJ|0000-0002-3571-501X']

2021

32517758

Characterization of clinical enterococci isolates, focusing on the vancomycin-resistant enterococci in a tertiary hospital in China: based on the data from 2013 to 2018.

BACKGROUND Vancomycin-resistant Enterococcus spp. (VRE) have spread all over the world. The present study aims to investigate the species distribution, specimen type and susceptibilities of Enterococcal species collected from Nanjing Drum Tower Hospital from 2013 to 2018. Additionally, distribution of VRE and prevalence of van gene among VRE isolates were also analyzed. METHODS The susceptibilities of 3913 Enterococcus isolates were retrospectively investigated. Among these strains, 60 VRE strains were further anazlyed in this study. The minimum inhibitory concentrations (MICs) of the VRE strains towards vancomycin, teicoplanin and linezolid were determined by E-test. Polymerase chain reaction (PCR) and DNA sequencing were used to investigate the prevalence of van genes among VRE. Furthermore, the sequence types (STs) of VRE strains were explored by multi-locus sequence typing (MLST). RESULTS Among the 3913 enterococci isolates, Enterococcus faecalis (n = 1870, 47.8%) and Enterococcus faecium (1738, 44.4%) were the main isolates. These Enterococcus strains were mainly isolated from urine (n = 1673, 42.8%), followed by secretions (n = 583, 14.9%) and ascites (n = 554, 14.2%). VRE displayed a decreasing trend year by year. Molecular analysis revealed that 49 out of 60 VRE isolates carried vanA gene, 10 carried vanM, and 1 carried both vanA and vanM genes. Sixteen distinct STs were identified among the 58 VREM, with ST78 (n = 16), ST192 (n = 8) and ST570 (n = 7) being the most dominant ones. CONCLUSIONS E. faecalis and E. faecium were the major enterococci strains which are the main pathogens of urinary traction infections; vanA and vanM were the main determinants conferring resistance to vancomycin; ST78, ST192 and ST570 were the leading STs of VREM which displayed a decreasing trend of prevalence year by year.

['Zhou|Wanqing|W|', 'Zhou|Hui|H|', 'Sun|Yuhan|Y|', 'Gao|Shuo|S|', 'Zhang|Yan|Y|', 'Cao|Xiaoli|X|', 'Zhang|Zhifeng|Z|', 'Shen|Han|H|', 'Zhang|Chunni|C|']

2020

31830655

Longitudinal dispersion of microplastics in aquatic flows using fluorometric techniques.

Microplastics are an emerging environmental contaminant. Existing knowledge on the precise transport processes involved in the movement of microplastics in natural water bodies is limited. Microplastic fate-transport models rely on numerical simulations with limited empirical data to support and validate these models. We adopted fluorometric principles to track the movement of both fluorescent dye and florescent stained microplastics (polyethylene) in purpose-built laboratory flumes with standard fibre-optic fluorometers. Neutrally buoyant microplastics behaved in the same manner as a solute (Rhodamine) and more importantly displayed classical fundamental dispersion theory in uniform open channel flow. This suggests Rhodamine, a fluorescent tracer, can be released into the natural environment with the potential to mimic microplastic movement in the water column.

['Cook|Sarah|S|', 'Chan|Hui-Ling|HL|', 'Abolfathi|Soroush|S|', 'Bending|Gary D|GD|', 'Schäfer|Hendrik|H|', 'Pearson|Jonathan M|JM|']

2020

32470877

Long noncoding RNA GAS5 promotes microglial inflammatory response in Parkinson's disease by regulating NLRP3 pathway through sponging miR-223-3p.

Parkinson's disease (PD) is the second most common neurodegenerative disorder. Neuroinflammation induced by microglia plays an important role in the pathogenesis of PD. Long noncoding RNA GAS5 was showed to have significant effects on regulating inflammatory response. Here, we aim to investigate the effects of GAS5 on the inflammatory response of PD, and the underlying mechanism. An in vivo model of PD was established in C57BL/6 mice by rotenone and an in vitro cell model was conducted on microglia by lipopolysaccharide (LPS). Our results indicated that GAS5 was upregulated in tissues in a mice model of PD and microglia activated by LPS. Gain- and loss- of functional experiments demonstrated that GAS5 promoted the inflammation of microglia in vitro. Besides, the knockdown of GAS5 repressed the PD progression in vivo. Mechanistically, GAS5 positively regulated the NLRP3 expression via competitively sponging miR-223-3p. Overall, our finding illuminates that GAS5 accelerates PD progression through targeting miR-223-3p/NLRP3 axis.

['Xu|Wei|W|', 'Zhang|Ling|L|', 'Geng|Yu|Y|', 'Liu|Ye|Y|', 'Zhang|Ning|N|']

2020

31396641

Size threshold for follow-up of postmenopausal adnexal cysts: 1 cm versus 3 cm.

OBJECTIVES To assess 3 cm size threshold for follow-up of simple cysts in postmenopausal women. MATERIALS AND METHODS Radiology information system was retrospectively queried for "US pelvis complete" over 8 years in women > 50 years, with keywords ovarian cyst, adnexal cyst, ovarian mass, cystic mass, cystic neoplasm, ovarian neoplasm, and ovarian mass. Premenopausal women were excluded. Cysts, were classified as ≤ 1 cm, 1-3 cm, 3-5 cm, and ≥ 7 cm. Largest cysts on each ovary was recorded. EMR and imaging archives were reviewed for assessing size, stability duration, and surgical records. Descriptive statistics and confidence interval were performed. RESULTS 4388 patients met the initial search criteria. 919 cysts in 896 women (age: 50-91 years, mean: 61.5 years) were identified. We found 162 cysts ≤ 1 cm, 352 1-3 cm, 296 3-7 cm , and 51 ≥ 7 cm cysts. 127 patients with 1-3 cm cysts had no follow-up. Final analysis of 225 1-3 cm cysts included 203 ovarian and 22 paraovarian cysts (average size = 1.95 cm (1.1-3.0 cm)). 103 ovarian cysts had less than 2 years, and 100 cysts had more than 2 years follow-up. All except one ovarian cyst were stable for the entire duration of their follow-up (Mean duration of follow-up 5.4 years) (0.3%, 95% CI 0.0-0.05). 40 cysts resolved. One simple cyst increased in size (followed over 3.25 years) without suspicious imaging features and benign on surgery. CONCLUSION 1-3 cm cysts represented the most common size range (> 40%) in postmenopausal women, majority of which are stable over follow-up with benign outcome. 3 cm size threshold is appropriate for simple cyst follow-up in postmenopausal women.

['Jha|Priyanka|P|http://orcid.org/0000-0001-5417-7723', 'Shekhar|Mallika|M|', 'Goldstein|Ruth|R|', 'Morgan|Tara|T|', 'Poder|Liina|L|']

2020

30836829

Association of serotoninergic pathway gene variants with elite athletic status in the Polish population.

Genetic factors are known to influence sport performance. The aim of the present study was to assess genetic variants in genes coding for proteins potentially modulating activity of brain emotion centres in a group of 621 elite athletes (212 endurance, 183 power and 226 combat athletes) and 672 sedentary controls. Ten statistically significant variants were identified in genes encoding elements of serotoninergic, catecholaminergic and hypothalamic-pituitary-adrenal systems in different sport groups. Of those the rs860573 variant in the FEV gene coding for transcription factor exclusively expressed in neurons of the central serotonin system is the only one whose frequency significantly differentiates all the groups of athletes studied, regardless of discipline, from the controls (p = 0.000026). Our results support the hypothesis that genetic variants potentially affecting mental processes and emotions, particularly in the serotonergic pathway, also influence the predispositions to athletic performance.

['Peplonska|Beata|B|http://orcid.org/0000-0002-6987-1993', 'Safranow|Krzysztof|K|', 'Adamczyk|Jakub|J|http://orcid.org/0000-0003-4558-1585', 'Boguszewski|Dariusz|D|', 'Szymański|Konrad|K|', 'Soltyszewski|Ireneusz|I|', 'Barczak|Anna|A|', 'Siewierski|Marcin|M|', 'Ploski|Rafal|R|', 'Sozanski|Henryk|H|', 'Zekanowski|Cezary|C|']

2019

25184976

Caesarean section rates in Southwestern Ontario: changes over time after adjusting for important medical and social characteristics.

OBJECTIVE To compare Caesarean section rates in a cohort of women in Southwestern Ontario over time, overall, and in patient subgroups defined by the Robson criteria, after adjusting for important medical and social characteristics. METHODS We obtained data from a perinatal database on deliveries at ≥ 22 weeks' gestation at a level II centre and a level III centre in London, Ontario between 1999 and 2010. Caesarean section rates were examined overall and in subgroups defined by parity, presentation, plurality, gestational age, and history of previous Caesarean section. Multivariable modified Poisson regression was used to compare Caesarean section rates in 2003-2006 and 2007-2010 versus 1999-2002. RESULTS In the fully adjusted models, the overall Caesarean section rate was significantly higher in 2007-2010 than in 1999-2002 for the level II centre (adjusted relative risk [aRR] 1.12; 95% CI 1.05 to 1.21). An increase was also seen in the level III centre in both 2003 to 2006 (aRR 1.19; 95% CI 1.14 to 1.24) and 2007 to 2010 (aRR 1.17; 95% CI 1.12 to 1.22). Similar increases were seen over time among patient subgroups. Notably, repeat Caesarean sections without labour increased at the level II centre (2003 to 2006 aRR 1.21; 95% CI 1.01 to 1.45, and 2007 to 2010 aRR 1.44; 95% CI 1.21 to 1.71) and the level III centre (2003 to 2006 aRR 1.72; 95% CI 1.53 to 1.94, and 2007 to 2010 aRR 1.77; 95% CI 1.57 to 2.00). CONCLUSION There has been a significant increase over time in the Caesarean section rate overall and in important subgroups. This increase remains even after controlling for other factors which may explain the trend.

['Brown|Hilary K|HK|', 'Hill|Jacquelyn|J|', 'Natale|Renato|R|']

2014

23896441

Effective removal of Congo red dye from aqueous solution using modified xanthan gum/silica hybrid nanocomposite as adsorbent.

The aim of this work is to study the feasibility of XG-g-PAM/SiO2 nanocomposite towards its potential application as high performance adsorbent for removal of Congo red (CR) dye from aqueous solution. The surface area, average pore size and total pore volume of the developed nanocomposite has been determined. The efficiency of CR dye adsorption depends on various factors like pH, temperature of the solution, equilibrium time of adsorption, agitation speed, initial concentration of dye and adsorbent dosage. It has been observed that the nanocomposite is having excellent CR dye adsorption capacity (Q0=209.205 mg g(-1)), which is considerably high. The dye adsorption process is controlled by pseudo-second order and intraparticle diffusion kinetic models. The adsorption equilibrium data correlates well with Langmuir isotherm. Desorption study indicates the efficient regeneration ability of the dye loaded nanocomposite.

['Ghorai|Soumitra|S|', 'Sarkar|Amit Kumar|AK|', 'Panda|A B|AB|', 'Pal|Sagar|S|']

2013

30870785

Newly identified interferon tau-responsive Hes family BHLH transcription factor 4 and cytidine/uridine monophosphate kinase 2 genes in peripheral blood granulocytes during early pregnancy in cows.

In cattle, interferon-stimulated genes (ISGs) such as ISG15, MX1, MX2, and OAS1 are known as classic ISGs that are highly involved in the implantation process. Various molecules play a crucial role in the mechanisms underlying ISG effects. Although microarray analyses have highlighted the expression of various molecules during the implantation period, these molecules remain incompletely characterized. In the present study, various specifically expressed genes were selected and their characteristics were examined. The microarray data from peripheral blood leukocytes derived from artificially inseminated cows and granulocytes obtained from embryo-transferred cows, respectively, were used to identify new ISG candidates. Seven common genes, including ISG15 and OAS1, were confirmed, but only 4 of the 5 genes were amplified by reverse transcription quantitative polymerase chain reaction. In addition, 3 expressed sequence tags (ESTs) exhibited significantly greater expression in granulocytes from pregnant cows than that observed in bred nonpregnant cows, and the expression in granulocytes increased after interferon-tau stimulation. Sequence alignment revealed similar sequences within 2 ESTs on the Hairy and enhancer of split (Hes) family basic helix-loop-helix transcription factor 4 (HES4) gene. An additional EST was identified as cytidine/uridine monophosphate kinase 2 (CMPK2). In silico analysis facilitated the identification of transcription factor-binding sequences, including an interferon-stimulated response element and interferon regulatory factor-binding sites, within the promoter region of HES4 and CMPK2. These genes may function as new ISGs in the context of implantation and may participate in the coordination of the feto-maternal interface in cows.

['Kikuchi|M|M|', 'Kizaki|K|K|', 'Shigeno|S|S|', 'Toji|N|N|', 'Ishiguro-Oonuma|T|T|', 'Koshi|K|K|', 'Takahashi|T|T|', 'Hashizume|K|K|']

2019

28833712

Cell-free fetal DNA analysis in maternal plasma as screening test for trisomies 21, 18 and 13 in twin pregnancy.

OBJECTIVES To evaluate in twin pregnancy the utility of non-invasive prenatal testing using circulating cell-free fetal DNA (cfDNA) in screening for the three main autosomal fetal trisomies. METHODS cfDNA testing was offered to 492 patients with a twin pregnancy without ultrasound anomaly as a first-line screening test or after routine serum screening. Data were collected prospectively and a retrospective analysis was performed. cfDNA analysis was performed by massively parallel sequencing. The fetal-fraction threshold used for test evaluation was 8%. Regression analysis was performed to investigate the effect on the test failure rate of maternal and pregnancy characteristics, and the performance of the test was also reported. RESULTS cfDNA analysis was performed as a first-line test (after the first-trimester scan) in 377 patients and following serum screening in 115. Of the 420 pregnancies for which outcome was available and cfDNA screening was assessed, 78.7% were dichorionic-diamniotic. The test failed on the first attempt in 12 (2.9%) pregnancies, and regression analysis demonstrated that only maternal weight was a significant independent predictor of test failure. A result was subsequently achieved in the 10 cases for which a second sample was obtained. cfDNA analysis identified all three cases of trisomy 21 and the only case of trisomy 18. For trisomy 21, the specificity was 99.8% (95% CI, 98.7-100.0%). When considering pregnancies according to whether they were conceived spontaneously or after assisted reproductive technology, there were no significant differences in terms of maternal weight or no-result rate for cfDNA screening between these two groups. CONCLUSIONS In twin pregnancy without fetal ultrasound abnormality, cfDNA screening for trisomies 21, 18 and 13 had a high success rate and good performance. Therefore, in routine practice, cfDNA analysis could be considered as a first- or second-line screening test. Copyright © 2017 ISUOG. Published by John Wiley & Sons Ltd.

['Le Conte|G|G|', 'Letourneau|A|A|', 'Jani|J|J|http://orcid.org/0000-0003-2252-342X', 'Kleinfinger|P|P|', 'Lohmann|L|L|', 'Costa|J-M|JM|', 'Benachi|A|A|http://orcid.org/0000-0001-6045-0765']

2018

30711254

Inhibitor mediated WNT and MEK/ERK signalling affects apoptosis and the expression of quality related genes in bovine in vitro obtained blastocysts.

Culture conditions determine embryo quality, which may be affected on many levels (timing of development, blastomere count, transcripts, metabolite content, apoptosis). Molecular interactions of signalling pathways like MEK/ERK and WNT/β-catenin are critical for cell-to-cell communication and cellular differentiation. Both pathways are important regulators of apoptosis. We have aimed to verify the prolonged effect of MEK/ERK silencing and WNT activation by chemical inhibitors (2i or 3i systems) on bovine IVP embryos. Apoptotic index, total cell count and transcription of embryo quality markers were evaluated. A higher rate of apoptosis was observed in 2i blastocysts, but was not accompanied by changes in transcript content of genes controlling apoptosis (BAX, BCL2, BAK, BAX/BCL2 ratio). Therefore, alternative pathways of apoptotic activation cannot be ruled out. The expression of genes related to embryo quality (HSPA1A, SLC2A1) was not affected. GJA1 transcripts were significantly higher in 3i blastocysts, what indicates a stimulatory effect of the applied inhibitors on cell-to-cell interactions. The lowest mRNA level of the IFNT2 gene was found in 2i embryos. A variation in the SDHA gene transcript was observed (with the highest content in the 3i blastocysts), what may suggest their reduced quality. It may be concluded that the modifications of culture conditions (activation of the WNT and silencing of the MEK/ERK signalling) might alter pathways crucial for embryo development without causing embryonic death.

['Madeja|Zofia E|ZE|', 'Warzych|Ewelina|E|', 'Pawlak|Piotr|P|', 'Lechniak|Dorota|D|']

2019

31465939

Development of an antibacterial nanobiomaterial for wound-care based on the absorption of AgNPs on the eggshell membrane.

To develop a promising antibacterial agent for wound-care dressing, a series of silver nanoparticles (AgNPs) and eggshell membrane (ESM) composites (AgNPs/ESM) were prepared. AgNPs were prepared using a chemical reduction method and their characteristics were determined. Various pH and processing time combinations were tested to find the optimal conditions for preparing AgNPs/ESM composites. To obtain the optimal nontoxic-level silver release, the AgNPs stock solution was diluted to 2 times, 4 times, 6 times, 8 times and 10 times with water and the concentration of silver released by the composites was also tested. All the prepared composites showed antibacterial activity, but the activity was the strongest when the stock AgNPs solution was diluted to four times (a concentration of silver was 2.41 mg/L). The addition of AgNPs changed the ESM from hydrophobic to hydrophilic by lowering the water contact angles from 105° to 75°, which is important for the wound-healing process. And the AgNPs/ESM composites had a higher surface area (159.08 m2/g) than the natural ESM (24.32 m2/g) and a suitable pore size (10.92 nm) as well, endowing with better absorption and antibacterial abilities. These findings suggested that the AgNPs/ESM composites are promising candidates for the development of antimicrobial agent for biomedical devices and therapeutic applications, such as wound-healing agent.

['Li|Xiaoyun|X|', 'Cai|Zhaoxia|Z|', 'Ahn|Dong Uk|DU|', 'Huang|Xi|X|']

2019

29019162

LsrB-based and temperature-dependent identification of bacterial AI-2 receptor.

The luxS gene is required for autoinducer-2 (AI-2) synthesis in many bacterial species. AI-2 is taken up by a specific receptor to regulate multiple bacterial activities. However, the lack of methods to identify AI-2 receptors has impeded investigations into the roles of AI-2. Here, a luxS mutant of Escherichia coli strain BL21 (DE3) was constructed (named BL21∆luxS), and the recombinant LsrB protein of Salmonella enterica was expressed in BL21∆luxS and BL21 cells, which were named LsrB (BL21∆luxS) and LsrB (BL21), respectively. The results of the activity of recombinant LsrB binding showed that LsrB (BL21) bound to endogenous AI-2 (produced from BL21 strain), while LsrB (BL21∆luxS) did not (as BL21∆luxS cannot produce AI-2). However, the results of recombinant LsrB binding showed that LsrB (BL21∆luxS) can bind exogenous AI-2, which was released from LsrB (BL21∆luxS) at 55 °C for 10 min, while LsrB (BL21) could not bind exogenous AI-2 (due to binding of endogenous AI-2 before). Furthermore, analysis of the thermal stability of AI-2 showed that that AI-2 activity was relatively high at incubation temperatures below 65 °C. These findings will be beneficial for screening of new AI-2 receptors in different bacterial species.

['Zhang|Yuxi|Y|', 'Qi|Kezong|K|', 'Jing|Yawei|Y|', 'Zuo|Jiakun|J|', 'Hu|Jiangang|J|', 'Lv|Xiaolong|X|', 'Chen|Zhaoguo|Z|', 'Mi|Rongsheng|R|', 'Huang|Yan|Y|', 'Yu|Shengqing|S|', 'Han|Xiangan|X|']

2017

31352846

Sepsis calculator for neonatal early onset sepsis - a systematic review and meta-analysis.

BACKGROUND Over investigation and overuse of empirical antibiotics is a concern in management of neonatal early onset sepsis (EOS) using the Centers for Disease Control and Prevention guidelines. "Sepsis calculator" is a risk-based prediction model for managing neonates at risk of EOS. OBJECTIVE To compare outcomes of neonatal EOS using of sepsis calculator versus conventional approach. METHODS A systematic review of randomized controlled trials (RCT) and non-RCTs reporting on outcomes after implementation of sepsis calculator for EOS for neonates >34-week gestation was conducted using the Cochrane methodology. Databases PubMed, CINAHL, Embase, Cochrane Central library and Google Scholar were searched in May 2019. Primary outcomes were antibiotics usage and laboratory tests for managing EOS. Secondary outcomes included hospital admissions and readmissions, blood culture positive EOS and mortality. The level of evidence (LOE) was summarized using the GRADE guidelines. RESULTS A total of 387 articles were retrieved after initial search. Six high quality non-RCTs fulfilled inclusion criteria. Meta-analysis (random effects model) showed that implementation of sepsis calculator was associated with reduced antibiotic usage [N = 172,385; OR = 0.22 (0.14-0.36); p < .00001; heterogeneity (I2) = 97%, Number needed to treat (NNT): 22], laboratory tests [N = 168,432; OR = 0.14 (0.08-0.27); p < .00001; I2 = 99%, NNT = 8], and admissions to neonatal unit [N = 16,628; OR = 0.24 (0.11-0.51); p = .0002; I2 = 98%, NNT = 7]; LOE: moderate. There was no difference in mortality, culture positive EOS, and readmissions. CONCLUSION Moderate quality evidence indicates that the implementation of a sepsis calculator was associated with reduced usage of antibiotics, laboratory tests and admission to neonatal unit with no increase in mortality and readmissions.

['Deshmukh|Mangesh|M|https://orcid.org/0000-0002-7485-4776', 'Mehta|Shailender|S|', 'Patole|Sanjay|S|']

2021

27959299

Drug-Induced Liver Injury.

Drug-induced liver injury (DILI) can result from both idiosyncratic and intrinsic mechanisms. This article discusses the clinical impact of DILI from a broad range of medications as well as herbal and dietary supplements. Risk factors for idiosyncratic DILI (IDILI) are the result of multiple host, environmental, and compound factors. Some triggers of IDILI often seen in critical care include antibiotics, antiepileptic medications, statins, novel anticoagulants, proton pump inhibitors, inhaled anesthetics, nonsteroidal anti-inflammatory agents, methotrexate, sulfasalazine, and azathioprine. The mechanism of IDILI due to these medications varies, and the resulting damage can be cholestatic, hepatocellular, or mixed. The primary treatment of IDILI is to discontinue the causative agent. DILI due to acetaminophen is intrinsic because the liver damage is predictably aligned with the dose ingested. Acute acetaminophen ingestion can be treated with activated charcoal or N-acetylcysteine. Future areas of research include identification of mitochondrial stress biomarkers and of the patients at highest risk for DILI.

['Hamilton|Leslie A|LA|', 'Collins-Yoder|Angela|A|', 'Collins|Rachel E|RE|']

2016

29345363

Effects of IL-10 and Th 2 cytokines on human Mφ phenotype and response to CSF1R inhibitor.

Tumor-associated Mφs display a plastic phenotype that is regulated by the local tumor milieu. Gene expression analysis and functional characterization of Mφs exposed in vitro to individual cytokines aids to delineate the cross-talk between defined cytokines shaping the complex Mφ phenotype. Human monocyte-derived Mφs can be differentiated in vitro with the T helper cell type 2 response cytokines IL-4 and IL-13 or the immunosuppressive IL-10. Notably, only the latter subset undergoes apoptosis when treated with the CSF 1 receptor (CSF1R) blocking antibody emactuzumab. However, under physiologic conditions, the Mφ phenotype is regulated by cytokine combination. Hence, in this study, we characterized the plasticity of IL-4 or IL-13-differentiated Mφs upon exposure to the immunosuppressive IL-10. Although IL-4-differentiated Mφs sustained their molecular phenotype in the presence of IL-10, IL-13-differentiated Mφs were skewed towards the IL-10 phenotype. Gene expression profiling revealed unique IL-4+IL-10 and IL-13+IL-10 Mφ signatures associated with up-regulation of canonical NF-κB or Wnt/β-catenin signaling pathways, respectively. Although IL-10 was able to alter the surface marker and gene expression profile of IL-13-differentiated Mφs, addition of IL-10 did not restore emactuzumab susceptibility. Combining NF-κB and Wnt/β-catenin signaling inhibitors with emactuzumab had no effect on viability. On average 3-5% of cancer patients overexpressed IL-4, IL-13, or IL-10 mRNA in silico. Although a small patient subset overexpressed IL-10+IL-13, IL-4+IL-10 lacked co-expression. In vitro characterization of CSF1R inhibitor-refractory Mφ phenotypes can support novel pharmacological approaches to specifically target these cells.

['Pradel|Leon P|LP|', 'Franke|Andreas|A|', 'Ries|Carola H|CH|']

2018

25899785

Targeting HER2 aberrations as actionable drivers in lung cancers: phase II trial of the pan-HER tyrosine kinase inhibitor dacomitinib in patients with HER2-mutant or amplified tumors.

BACKGROUND HER2 mutations and amplifications have been identified as oncogenic drivers in lung cancers. Dacomitinib, an irreversible inhibitor of HER2, EGFR (HER1), and HER4 tyrosine kinases, has demonstrated activity in cell-line models with HER2 exon 20 insertions or amplifications. Here, we studied dacomitinib in patients with HER2-mutant or amplified lung cancers. PATIENTS AND METHODS As a prespecified cohort of a phase II study, we included patients with stage IIIB/IV lung cancers with HER2 mutations or amplification. We gave oral dacomitinib at 30-45 mg daily in 28-day cycles. End points included partial response rate, overall survival, and toxicity. RESULTS We enrolled 30 patients with HER2-mutant (n = 26, all in exon 20 including 25 insertions and 1 missense mutation) or HER2-amplified lung cancers (n = 4). Three of 26 patients with tumors harboring HER2 exon 20 mutations [12%; 95% confidence interval (CI) 2% to 30%] had partial responses lasting 3+, 11, and 14 months. No partial responses occurred in four patients with tumors with HER2 amplifications. The median overall survival was 9 months from the start of dacomitinib (95% CI 7-21 months) for patients with HER2 mutations and ranged from 5 to 22 months with amplifications. Treatment-related toxicities included diarrhea (90%; grade 3/4: 20%/3%), dermatitis (73%; grade 3/4: 3%/0%), and fatigue (57%; grade 3/4: 3%/0%). One patient died on study likely due to an interaction of dacomitinib with mirtazapine. CONCLUSIONS Dacomitinib produced objective responses in patients with lung cancers with specific HER2 exon 20 insertions. This observation validates HER2 exon 20 insertions as actionable targets and justifies further study of HER2-targeted agents in specific HER2-driven lung cancers. CLINICALTRIALSGOV NCT00818441.

['Kris|M G|MG|', 'Camidge|D R|DR|', 'Giaccone|G|G|', 'Hida|T|T|', 'Li|B T|BT|', "O'Connell|J|J|", 'Taylor|I|I|', 'Zhang|H|H|', 'Arcila|M E|ME|', 'Goldberg|Z|Z|', 'Jänne|P A|PA|']

2015